By means of field investigation and specimen identification, four genera and nine species in Orchidaceae are reported as new records in Yunnan Province. Nine species are Biermannia calcarata Aver., Cyrtosia septentrionalis (Rchb. f.) Garay, Cyrtosia nana ( Rolfe ex Downie) Garay, Didymoplexiella siamensis ( Rolfe ex Downie ) Seidenf., Didymoplexiopsis khiriwongensis Seidenf., Epipogium kentingensis T. P. Lin et S. H. Wu, Eria thao Gagnep., Lecanorchis nigricans Honda and Liparis barbata Lindl. Four genera are Biermannia King et Pantl., Cyrtosia Blume, Didymoplexiella Garay and Didymoplexiopsis Seidenf. Voucher specimens are deposited in Herbarium of Xishuangbanna Tropical Botanical Garden (HITBC).