2024年7月17日 星期三
转天麻抗真菌蛋白GAFP基因烟草的获得及离体抑菌活性检测
Acquirement of tabacco with transformation genes of GAFP ( Gastrodia Antifungal Protein) and evalution of antifungal activity in vitro
2002年 第11卷 第2期 页码[1-5]    下载全文[0.6MB]  
摘要

通过根癌农杆菌( Agrobacterium tumefaciens) 介导, 采用叶盘法将天麻( Gastrodia elata Bl .) 抗真菌蛋白基因GAFP
转入烟草( Nicotiana tabacum L.) , PCR 和 Southern-blotting 分析证明已将 GAFP 基因整合进烟草植株。 体外抑菌实验
表明GAFP 转基因的烟草植株对真菌瑞氏木霉( Trichoderma reesei) 表现出一定的抑菌活性。
 

Abstract

Mediated by Agrobacterium tumefaciens, the gene of Gastrodia Antifungal Protein ( GAFP) was transferred into tobacco ( Nicotiana tabacum L. ) using the leaf-disk method.Results of PCR and Southernblotting proved that GAFP gene was integrated into the tobacco genome. Extracts of transgenic lines showed resistance to the growth of Trichoderma reesei in vitro, which suggests that the GAFP gene was expressed efficiently in transgenic tobacco and denoted antifungal activity.

关键词根癌农杆菌介导; 天麻抗真菌蛋白; 转基因烟草; 离体抑菌活性;
Key wordsAgrobacterium tumefaciens mediated transformation; Gastrodia Antifungal Protein; transgenic tobacco; antifungal activity in vitro
作者陈 英1, 王义琴2, 诸葛强1, 施季森1, 黄敏仁1, 王明庥1
所在单位1.南京林业大学林木遗传与基因工程重点实验室, 江苏 南京, 210037;
2.中国科学院遗传与发育生物研究所, 北京, 100101
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基金项目国家自然科学基金农业倾斜项目( 3017074)