2021年12月6日 星期一
抗赖氨酸加苏氨酸莲体细胞变异体的筛选
Selection of somaclonal variants of lotus ( Nelumbo nucifera Gaertn. ) by lysine plus threoine stressing
1998年 第7卷 第1期 页码[15-19]    下载全文[0.6MB]  
摘要

对籽莲红花建莲( Nelumbo nucifera cv .Hong huajianlian) 和白心湘莲( N .nucifera cv.Baixinx ianglian) 杂交而成的幼胚的子叶胚轴及幼胚叶( ) 形成的愈伤组织, 施以赖氨酸加苏氨酸胁迫培养 30 , 从中筛选出抗性愈伤组织并形成再生植株低浓度的赖氨酸加苏氨酸促进愈伤组织的生长, 而高浓度的赖氨酸加苏氨酸则抑制愈伤组织生长, 直至具有致死作用, 这种致死作用
是因为高浓度的赖氨酸加苏氨酸抑制了天冬氨酸合成途径中的天冬氨酸激酶和高丝氨酸脱氢酶造成的
, 细胞发生变异后对赖氨酸和苏氨酸产生了抗性, 即与天冬氨酸合成途径有关的氨基酸增抗性愈伤组织与未胁迫的愈伤组织的氨基酸测定表明, 在总计 17 种氨基酸中, 抗性愈伤组织14 种氨基酸含量超过原始愈伤组织, 1 种持平, 2 种不及原始愈伤组织再生抗性植株的莲籽氨基酸测定显示, 17 种氨基酸中, 12 种超过母本红花建莲, 15 种超过父本白心湘莲
 

Abstract

Callus induced from young embry os hybrided by Nelumbo nucifera cv .Honghuajianlian
× N .nucifera cv .Baixinxianglian were stressed by lysine plus threoine for 30 days
.Resistant callus had been selected in vitro and regenerated into plants .Results showed that low concentration of lysine plus threoine could promote the growth of callus, whereas high concentration prohibited their grow th, even lethal to callus . Determination of amino acids of resistant callus showed that of all 17 amino acids, 14 more than, 1 equal to and 2 less than those in normal callus .Amino acid analysis of seeds of variant indicated that of all 17 amino acids, 12 in variant was more than in N . nucifera cv .Honghuajianlian and 15 in them was more than those in N .nucifera cv .Baixinxianglian .