It was first reported that leaf explants of Rubus rosaefolius w ere cultured in vitro on MS medium containing BA 1 mg/l , IAA 0.1 mg./l , GA₃ 0. 2 mg/l. Adventitious buds formed directly from segments of leaf top, leaf base and petiole. About 45% of the explants formed buds. The medium for adventitious bud formation was the same as it for bud proliferation.