应用正交实验研究了生长调节物质病毒唑( ribavirin) 、茉莉酸甲酯( jasmonic acid methylester) 、油菜素内酯( brassinolide) 、腐殖酸钠( sodium humate) 对组织 培养暗紫贝母( Fritillaria unibracteata Hisao et K.C.Hsia) 小鳞茎生长的影响, 发现病毒唑有显著活性。 进一步对不同浓度病毒唑的效果进行优化试验, 结果表明:病毒唑 10 mg/ L 是提高小鳞茎生长率的最适浓度。
 

Growth regulators including ribavirin, jasmonic acid methyl ester, brassinolide and sodium humate were added into the media with a view to determine their effect on cultured bulblets of Fritillaria unibracteata Hisao et K .C .Hsia.Results from the orthogonal experiments indicated that ribavirin had sig nificant promotive activity. Further optimizing experiment on the effect of different concentration of ribavirin showed that 10 mg/L ribavirin was the optimized concentration in promoting growth of induced bulblets .