Taking anthocyanin 1 and anthocyanin 2 isolated by activity orientation from fruit extracts of Lycium ruthenicum Murr. as materials， their inhibition rates on tyrosinase monophenolase and tyrosinase diphenolase activities were compared， and their structural identification and molecular docking research with tyrosinase were conducted. The results show that the inhibitory effect of anthocyanin 1 on tyrosinase activity is stronger， its semi-inhibitory concentration on tyrosinase monophenolase and tyrosinase diphenolase activities is 25.44 and 153.10 μg·mL^-1， respectively. Anthocyanin 1 and anthocyanin 2 are petunia-3-O-（6′′′′-O-trans-p-coumaroyl） rutinoside-5-O-glucoside and petunia-3-O-［6′′′′-O-（4′′′′′-O-glucosyl） trans-p-coumaroyl）］ rutinoside-5-O-glucoside， respectively. The binding ability of anthocyanin 1 with tyrosinase is stronger， its binding energy is -7.5 kcal·mol^-1. In conclusion， anthocyanin 1 has a stronger inhibitory effect on tyrosinase activity， so it can be used as an effective component of whitening products.