2021年10月17日 星期日
黑果枸杞果实提取物中花色苷对酪氨酸酶活性的抑制作用初探
Preliminary study on inhibitory effect of anthocyanins from fruit extracts of Lycium ruthenicum on tyrosinase activity
2020年 第29卷 第3期 页码[75-77]    下载全文[2.3MB]  
Abstract

Taking anthocyanin 1 and anthocyanin 2 isolated by activity orientation from fruit extracts of Lycium ruthenicum Murr. as materials, their inhibition rates on tyrosinase monophenolase and tyrosinase diphenolase activities were compared, and their structural identification and molecular docking research with tyrosinase were conducted. The results show that the inhibitory effect of anthocyanin 1 on tyrosinase activity is stronger, its semi-inhibitory concentration on tyrosinase monophenolase and tyrosinase diphenolase activities is 25.44 and 153.10 μg·mL-1, respectively. Anthocyanin 1 and anthocyanin 2 are petunia-3-O-(6′′′′-O-trans-p-coumaroyl) rutinoside-5-O-glucoside and petunia-3-O-[6′′′′-O-(4′′′′′-O-glucosyl) trans-p-coumaroyl)] rutinoside-5-O-glucoside, respectively. The binding ability of anthocyanin 1 with tyrosinase is stronger, its binding energy is -7.5 kcal·mol-1. In conclusion, anthocyanin 1 has a stronger inhibitory effect on tyrosinase activity, so it can be used as an effective component of whitening products.

关键词黑果枸杞; 果实; 花色苷; 酪氨酸酶活性; 抑制作用
Key wordsLycium ruthenicum Murr.; fruit; anthocyanin; tyrosinase activity; inhibitory effect
作者黄佳楠1, 管福琴1, 李林蔚1, 李晓莺2, 禄璐2, 米佳2, 刘兰英2, 陈雨1
所在单位1. 江苏省中国科学院植物研究所(南京中山植物园) 江苏省植物资源研究与利用重点实验室 江苏省农业种质资源保护与利用平台, 江苏 南京 210014; 2. 宁夏农林科学院枸杞工程技术研究所, 宁夏 银川 750002
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基金项目宁夏农林科学院对外科技合作专项(DW-X-2018014; DW-X-2018009); 国家自然科学基金青年基金项目(31401898)