2018年9月23日 星期日
聚多曲霉菌对Cd胁迫下芥菜种子萌发、生长和Cd吸收的影响
Effect of Aspergillus sydowii on seed germination, growth, and Cd uptake of Brassica juncea under Cd stress
2018年 第27卷 第3期 页码[25-32]
摘要

为了探明聚多曲霉菌〔Aspergillus sydowii (Bain. et Sart.) Thom et Church〕对芥菜〔Brassica juncea (Linn.) Czern.〕种子萌发、生长和Cd吸收的影响,以聚多曲霉菌菌株DJ515-2为供试菌株,对1 mmol·L-1 Cd胁迫下接菌组(接种浓度为1.0×108 CFU·mL-1的DJ515-2菌悬液)和对照组(未接种DJ515-2菌悬液)芥菜的种子萌发状况进行了比较,并采用盆栽法对0、28、56和112 mg·kg-1Cd胁迫下接菌组和对照组芥菜单株地上部和地下部的鲜质量和干质量、地上部和地下部的Cd含量、单株Cd吸收量及根际土壤有效态Cd含量进行了比较。结果表明:与对照组相比,接菌组的种子发芽率、根长、茎长和种子活力指数分别升高了20.0%、52.4%、56.3%和83.7%。在不同质量浓度Cd胁迫下,接菌组的单株地上部和地下部的鲜质量和干质量、地上部和地下部的Cd含量、单株Cd吸收量及根际土壤有效态Cd含量均高于对照组;并且,除地上部Cd含量外,28 mg·kg-1Cd胁迫下接菌组的其他指标均显著(P<0.05)高于对照组。随着Cd质量浓度的提高,对照组和接菌组的单株地上部鲜质量及单株地上部和地下部的干质量总体上逐渐降低,地上部和地下部的Cd含量、单株Cd吸收量及根际土壤有效态Cd含量总体上逐渐升高。研究结果显示:聚多曲霉菌菌株DJ515-2能够促进芥菜种子萌发、生长和Cd吸收,并能提高土壤中Cd的生物有效性,对芥菜修复低浓度(28 mg·kg-1)Cd污染土壤具有明显的促进作用。

 

Abstract

In order to explore the effect of Aspergillus sydowii (Bain. et Sart.) Thom et Church on seed germination, growth, and Cd uptake of Brassica juncea (Linn.) Czern., taking A. sydowii strain DJ515-2 as tested strain, seed germination status of B. juncea in inoculation group (inoculating DJ515-2 fungal suspension with concentration of 1.0×108 CFU·mL-1) and the control group (not inoculating DJ515-2 fungal suspension) under 1 mmol·L-1 Cd stress was compared, and fresh and dry weights of above- and under-ground parts per plant, Cd content in above and underground parts, Cd uptake per plant, and available Cd content in rhizosphere soil of B. juncea in inoculation group and the control group under 0, 28, 56, and 112 mg·kg-1 Cd stresses were compared by using pot method. The results show that compared with the control group, seed germination rate, root length, stem length, and seed vigor index in inoculation group increase by 20.0%, 52.4%, 56.3%, and 83.7%, respectively. Under different mass concentrations of Cd stress, fresh and dry weights of above- and under-ground parts per plant, Cd content in above- and under-ground parts, Cd uptake per plant, and available Cd content in rhizosphere soil in inoculation group are all higher than those in the control group; and except Cd content in above-ground part, all other indexes in inoculation group under 28 mg·kg-1 Cd stress are significantly (P<0.05) higher than those in the control group. With increasing of Cd mass concentration, fresh weight of aboveground part per plant and dry weight of above- and under-ground parts per plant in the control group and inoculation group decrease gradually in general, and Cd content in above- and under-ground parts, Cd uptake per plant, and available Cd content in rhizosphere soil increase gradually in general. It is suggested that A. sydowii strain DJ515-2 can promote seed germination, growth and Cd uptake of B. juncea, and can improve the bioavailability of Cd in soil, shows an obviously promotive effect on restoration of low concentration (28 mg·kg-1) Cd contaminated soil by B. juncea.
 

关键词聚多曲霉菌; 芥菜; 种子萌发; 生长; Cd吸收量; 植物修复
Key wordsAspergillus sydowii (Bain. et Sart.) Thom et Church; Brassica juncea (Linn.) Czern.; seed germination; growth; Cd uptake; phytoremediation
作者王涛1, 张海利1, 邹路易1, 郁红艳1, 黄振兴1,2, 滕跃1
所在单位1. 江南大学环境与土木工程学院 江苏省厌氧生物技术重点实验室, 江苏 无锡 214122;2. 江苏省水处理技术与材料协同创新中心, 江苏 苏州 215009
点击量44
基金项目国家自然科学基金资助项目(21307043); 中国博士后科学基金面上资助项目(2016M590411)