2022年1月21日 星期五
5种苍术属药用植物的trnL-F序列测定及种间遗传关系分析
Sequencing of trnL-F and analysis of interspecific genetic relationship of five medicinal species in Atractylodes DC.
2007年 第16卷 第2期 页码[12-16]    下载全文[0.7MB]  
摘要

采用PCR技术对5种苍术属(Atractylodes DC.)药用植物的trnL-F序列进行测定及比较分析。结果表明,扩增获得的序列长886~902 bp,经排序并两端切平后,序列长816 bp,G+C含量为34.4%;当空位始终做缺失处理时,产物有5个变异位点。利用trnL-F序列可准确鉴别出朝鲜苍术〔A. koreana(Nakai) Kitam.〕、北苍术〔A. chinensis (DC.)Koidz.〕和白术(A. macrocephala Koidz.);而茅苍术〔A. lancea (Thunb.)DC.〕与关苍术(A. japonica Koidz. ex Kitam.)的trnL-F序列则完全一致,相互之间无法区分。采用邻接法和UPGMA法得到的5种苍术属药用植物的分子系统树有一定的差异,表明这5种苍术属药用植物的物种分化不完全,序列进化不一致。 

Abstract

The trnL-F sequences of five medicinal species in Atractylodes DC. Were analyzed by PCR technique. The results showed that the length of trnL-F sequence was 886- 902bp, and the length became to 816bp after aligned these sequences and cut the over hanging terminals. The percentage of G+C content was 34.4%. When the gaps were always treated as missing, there were five variable sites. Based on trnL-F sequences, A. koreana (Nakai)Kitam., A. chinensis (DC.) Koidz. And A. macrocephala Koidz. Could be identified accurately. But no difference between the trnL-F sequences of A. lancea (Thunb.)DC. and A. japonica Koidz. Ex Kitam., so these two species could not be distinguished from each other. The molecular phylogenetic trees of these five species which constructed by N-J and UPGMA methods were considerable discrepant It is suggested that species differentiation of these five medicinal species in Atractylodes DC. Are incomplete and the evolution of DNA sequence is not identical.

关键词苍术属; 叶绿体基因组; trnL-F; 遗传关系; 分子鉴别;
Key wordsAtractylodes DC.; cpDNA; trnL-F; genetic relationship; molecular identification
作者葛燕芬,杭悦宇,夏 冰,韦阳连
所在单位江苏省•中国科学院植物研究所(南京中山植物园)江苏省植物迁地保护重点实验室,江苏南京210014
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基金项目江苏省自然科学基金重点项目(BK2005206); 国家自然科学基金资助项目(30370292);