2023年2月1日 星期三
苍术 DNA 分离及 RAPD 遗传多样性分析
Isolation of DNA from Atractylodes lancea (Thunb .) DC.and genetic diversity
1997年 第6卷 第4期 页码[1-6]    下载全文[0.8MB]  
摘要

用新鲜的或-75 保存的苍术Atractylodes lancea ( Thunb .) DC .〕叶片提取 DNA, 产量分别为 40 ng/ mg 鲜重和 10 ng/mg 鲜重左右, 分离出的 DNA OD260/ OD280值均大于 1.9, 可用于RA PD 试验8 种引物对苍术 4 个居群的 DNA 进行扩增, 单个 10 碱基的引物扩增出的 RAPD标记在1 15 之间, 多态位点百分率南苍术为 47.50%, 北苍术为 45.40%, 遗传相似度值南苍术为0.85, 北苍术为 0.87 结果表明, 南苍术的遗传多样性略高于北苍术
 

Abstract

The DNA of fresh or stored ( under -75 ) leaves of Atractylodes lancea ( Thunb .) DC .were isolated, the yield is about 40 ng/mg FW and 10 ng/mg FW separately .The OD260/OD280 values of isolated DNA are all above 1 .9, the DNA can be used in the test of RAPD .The amplified results of 4 populations by 8 primers indicated that single 10-base oligonucleotide primer can produce 1 to 15 pieces of DNA fragments .The percentage of polymorphic loci are 47 .50 % in south A .lancea and 45 .40 %in the north ones, the genetic similarity value is 0 .85 in the former and 0 .87 in the later, it showed that the genetic diversity level in the south A . lancea is slightly higher .
 

关键词苍术; RAPD; 遗传多样性;
Key wordsAtracty lodes lancea (Thunb .)DC .; RAPD; genetic diversity
作者任冰如, 於 虹 ,贺善安
所在单位江苏省中国科学院植物研究所, 江苏省植物迁地保护重点实验室, 南京 210014
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基金项目国家自然科学基金资助项目和中日苍术资源与种质评价合作研究项目