对黄独(Dioscorea bulbifera L.)14个居群42个单株的总DNA进行RAPD分析,并根据测序结果合成1对特异的SCAR引物,即5′-GGCTTCTGTCACTACATGGG-3′和5′-GGCTTCTGTCCAGTGCATCT-3′,对42个单株的总DNA进行检测,扩增到1条长681 bp的与雄性相关的特异片段。除形态上有一定变异的4个单株外,这个与雄性相关的RAPD-SCAR标记在黄独原变种的29个单株中验证准确,在可能为其变种的9个单株中部分验证准确,表明该RAPD-SCAR标记能够准确标记黄独的原变种及一些变种的雄性性别。 

The total DNA of 42 individuals in fourteen populations of Dioscorea bulbifera L. were analyzed by RAPD technique. According to the obtained sequences, a pair of 20-mer specific primers,i.e. 5-GGCTTCTGTCACTACATGGG-3′and 5-GGCTTCTGTCCAGTGCATCT-3, were designed and synthesized as SCAR primers. The total DNA of 42 individuals were tested by these SCAR primers. A male-associated fragment about 681bp was amplified. The diagnostic reliability of male-associated RAPD-SCAR specific marker was verified on all 29 individuals of D. bulbifera var. bulbifera except four individuals which had the morphological variation, and some of nine individuals which might be varieties of D. bulbifera. It is indicated that the RAPD-SCAR specific marker can be used correctly for screening male gender of D. bulbifera var. bulbifera and some other varieties of it.