2019年10月17日 星期四
模拟酸雨处理后番茄叶片叶绿素含量及叶绿素荧光参数的动态变化
Dynamic changes in chlorophyll content and chlorophyll fluorescence parameters of leaf of Lycopersicon esculentum after simulated acid rain treatment
2019年 第28卷 第3期 页码[108-116]
摘要

 以番茄品种‘倍盈’(Lycopersicon esculentum ‘Beiying’)幼苗为研究材料,对pH 5.6(CK)、pH 3.5和pH 3.0模拟酸雨处理结束后0~20 d叶片的叶绿素含量、OJIP曲线和叶绿素荧光参数的动态变化进行了比较和分析。结果表明:在模拟酸雨处理结束后0~20 d,随时间延长,各叶绿素含量相关指标以及各叶绿素荧光参数均呈波动的变化趋势,其中,叶绿素荧光参数Fo、Vj、Mo、φDo、ABS/RC、TRo/RC、DIo/RC、TRo/CSo、DIo/CSo和RC/CSo值整体呈下降的趋势,Fv/Fo、Sm、ψo、φEo、ETo/RC、ETo/CSo值整体呈上升的趋势。在模拟酸雨处理结束后0~20 d,2个模拟酸雨处理组的叶绿素a、叶绿素b和总叶绿素含量以及pH 3.0模拟酸雨处理组的叶绿素a含量与叶绿素b含量的比值(Chla/Chlb)总体上低于CK组,其中,叶绿素b含量以及Chla/Chlb值在各组间均无显著差异;在pH 3.5模拟酸雨处理结束后5~20 d,叶绿素a和总叶绿素含量与CK组均无显著差异;而在pH 3.0模拟酸雨处理结束后0~20 d,叶绿素a和总叶绿素含量均显著低于CK组。在模拟酸雨处理结束后0 d,2个模拟酸雨处理组OJIP曲线的O和J值均高于CK组,P值则低于CK组,且曲线形状与CK组差异明显;但随时间延长,2个模拟酸雨处理组OJIP曲线的O和J值呈下降趋势,而I和P值则呈上升趋势,曲线形状与CK组趋同。与CK组相比,pH 3.0模拟酸雨处理结束后0 d,叶片Fm、Fv/Fo、Fv/Fm、Sm、ψo、φEo、ETo/RC和ETo/CSo值显著下降,Fo、Vj、Mo、φDo、ABS/RC、DIo/RC和DIo/CSo值显著上升,但pH 3.5模拟酸雨处理后叶片的大多数叶绿素荧光参数与CK组无显著差异。总体上看,在pH 3.5模拟酸雨处理结束后20 d,叶绿素含量基本恢复至CK组水平,且叶绿素荧光参数也在处理结束后0 d基本恢复至CK组水平;而在pH 3.0模拟酸雨处理结束后20 d,叶绿素含量未能恢复至CK组水平,且叶绿素荧光参数在处理结束后5~10 d逐渐恢复至CK组水平。综合分析结果表明:经模拟酸雨处理后,番茄叶片叶绿素a含量降低,OJIP曲线形状改变,PSⅡ反应中心受损,导致大多数叶绿素荧光参数发生改变,从而对光合系统产生影响;且随模拟酸雨pH值的降低,对叶绿素含量及叶绿素荧光参数的影响效应增大。

Abstract

Taking seedlings of Lycopersicon esculentum ‘Beiying’ as research material, dynamic changes in chlorophyll content, OJIP curve and chlorophyll fluorescence parameters of leaf were compared and analyzed after stopping simulated acid rain treatment at pH 5.6 (CK), pH 3.5 and pH 3.0 for 0-20 d. The results show that after stopping simulated acid rain treatment for 0-20 d, each chlorophyll content related index and chlorophyll fluorescence parameter all show a fluctuation change tendency with elongation of time, in which, values of fluorescence parameters of Fo, Vj, Mo, φDo, ABS/RC, TRo/RC, DIo/RC, TRo/CSo, DIo/CSo and RC/CSo show a tendency to decrease in general, while values of Fv/Fo, Sm, ψo, φEo, ETo/RC and ETo/CSo show a tendency to increase in general. After stopping simulated acid rain treatment for 0-20 d, contents of chlorophyll a, chlorophyll b and total chlorophyll of two simulated acid rain treatment groups, and ratio of chlorophyll a content to chlorophyll b content (Chla/Chlb) of pH 3.0 simulated acid rain treatment group are lower than those of CK group in general, in which, there is no significant difference in chlorophyll b content and Chla/Chlb value among each group. After stopping simulated acid rain treatment at pH 3.5 for 5-20 d, contents of chlorophyll a and total chlorophyll are not significantly different with those of CK group; while after stopping simulated acid rain treatment at pH 3.0 for 0-20 d, contents of chlorophyll a and total chlorophyll are significantly lower than those of CK group. After stopping simulated acid rain treatment for 0 d, O and J values of OJIP curves of two simulated acid rain treatment groups are all higher than those of CK group, while their P values are lower than those of CK group, and their curve shapes are significantly different with those of CK group; but with elongation of time, O and J values of OJIP curves of two simulated acid rain treatment groups show a tendency to decrease, while their I and P values do a tendency to increase, and their curve shapes are almost the same with those of CK group. Compared with CK group, values of Fm, Fv/Fo, Fv/Fm, Sm, ψo, φEo, ETo/RC and ETo/CSo of leaf decrease significantly, while values of Fo, Vj, Mo, φDo, ABS/RC, DIo/RC and DIo/CSo increase significantly after stopping simulated acid rain treatment at pH 3.0 for 0 d, but most chlorophyll fluorescence parameters of leaf are not significantly different with those of CK group after simulated acid rain treatment at pH 3.5. In general, after stopping simulated acid rain treatment at pH 3.5 for 20 d, chlorophyll content basically restores to the level of CK group, and chlorophyll fluorescence parameters basically restore to the level of CK group after stopping treatment for 0 d; while after stopping simulated acid rain treatment at pH 3.0 for 20 d, chlorophyll content cannot restore to the level of CK group and chlorophyll fluorescence parameters gradually restore to the level of CK group after stopping treatment for 5-10 d. The comprehensive analysis result shows that after simulated acid rain treatment, chlorophyll a content in leaf of L. esculentum decreases, shape of OJIP curve changes, and PSⅡ reaction center is damaged, resulting in variation of most chlorophyll fluorescence parameters, thus affecting photosynthesis system; with decrease of pH value of simulated acid rain, influences on chlorophyll content and chlorophyll fluorescence parameters increase.

关键词番茄; 模拟酸雨; 叶绿素含量; OJIP曲线; 叶绿素荧光参数
Key wordsLycopersicon esculentum Mill.; simulated acid rain; chlorophyll content; OJIP curve; chlorophyll fluorescence parameters
作者陈文胜1,2, 出佳范2, 吕再辉2, 黄晓松2, 徐冰莹2, 邱栋梁2
所在单位1. 福建农业职业技术学院园艺园林系, 福建 福州 350119; 2. 福建农林大学园艺学院, 福建 福州 350002
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基金项目福建省教育厅访问学者资助项目(闽教人[2013]60号); 福建农林大学科技创新专项基金项目(CXZX2016107)