以丰水梨(Pyrus pyrifolia‘Hosui’)离体叶片为实验材料,研究了氨苄青霉素(Amp)、羧苄青霉素(Carb)和头孢霉素(Cef)对丰水梨叶片不定芽再生的影响及其对根癌农杆菌(Agrobacterium tumefaciens)的抑菌效果。结果表明,在100~500 mg.L^-1浓度范围内,3种抗生素对丰水梨叶片愈伤组织诱导均无显著影响。Amp、Carb和Cef抑制丰水梨叶片不定芽再生的起始浓度分别为100、200和200 mg.L^-1;当浓度高达500 mg.L^-1时,Amp、Carb和Cef均极显著地抑制叶片不定芽再生。除500 mg.L^-1Cef处理组外,不同浓度Amp、Carb和Cef对丰水梨每个外植体的再生不定芽数没有显著影响。与对照相比,在100~500 mg.L^-1浓度范围内,3种抗生素均对根癌农杆菌菌株LBA4404和EHA105有显著的抑制作用,且对菌株EHA105的抑制效果强于菌株LBA4404。研究结果显示,在丰水梨遗传转化体系中,可以选择300 mg.L^-1的Amp和Cef作为理想的杀菌剂。 

Effects of ampicillin, carbenicillin and cefotaxime on regeneration of adventitious buds from Pyrus pyrifolia `Hosui' leaf and inhibition of the three antibiotics to Agrobacterium tumefaciens were studied within vitro leaves of P. pyrifolia `Hosui'. The results show that the three antibiotics (100- 500 mg·L^{- 1})have no significant effect on callus induction of the leaves. Adventitious bud regeneration starts to be restrainedat 100mg·L^{- 1}of ampicillin and 200mg·L^{- 1}of carbenicillin or cefotaxime. When the concentrations of the three antibiotics are up to 500mg·L^-1, there generation of adventitious buds are extremely significantly inhibited. Ampicillin, carbenicillin and cefotaxime with different concentrations have no significant effects on the regenerated adventitious bud number per explant except 500mg·L^-1 cefotaxime. Contrast to the control, reproduction of A. tumefaciens LBA 4404 and EHA 105 strains is significantly inhibited by the three antibiotics at 100-500mg·L^{- 1}, and the inhibition to EHA 105 is better than that to LBA 4404. It is concluded that300mg·L^{- 1}ampicillin and cefotaxime are ideal bactericides for P. pyrifolia `Hosui' genetictrans  formation system.