2022年1月20日 星期四
基于SSR 标记的8 个山荆子居群遗传多样性和遗传关系分析
Analyses of genetic diversity and genetic relationship of eight populations of Malus baccata based on SSR marker
2012年 第21卷 第1期 页码[42-46]    下载全文[0.6MB]  
摘要

采用10 对SSR 引物对8 个山荆子[Malus baccata (L.) Borkh.]页居群140 个单株的基因组总DNA 进行PCR 扩增,并据此对8 个居群的遗传多样性和遗传关系进行了分析。结果表明:用10 对SSR 引物共扩增出91 条带,多态性条带百分率达100. 00%。8 个居群的遗传多样性参数差异较大, 有效等位基因数为1. 437 9 ~1. 535 0, Nei’s 基因多样性指数为0. 256 0 ~0. 309 2,Shannon 信息指数为0. 376 7 ~ 0. 459 2, 多态性条带百分率为64. 84% ~85. 71%。居群间的有效等位基因数为1. 616 9,Nei’s 基因多样性指数为0. 355 1,Shannon 信息指数为0. 528 5,均明显高于居群内;8 个居群间的基因流为1. 739 5,基因分化系数为0. 223 3,显示居群间的基因交换较多。UPGMA聚类分析结果表明:在Nei’s 遗传距离0. 148 6 处,8 个居群被分为3 组,河北塞罕坝居群单独为一组,山西五台山居群和北京东灵山居群为一组,其余5 个居群为一组。据此推测:山荆子起源于中国华北和东北地区,山西灵空山、黑龙江小兴安岭、吉林长白山和山西中条山居群可能是其遗传多样性的核心居群。

Abstract

PCR amplification of total genomic DNA from 140 individuals of eight populations of Malus baccata (L.) Borkh. was carried out by using ten pairs of SSR primers, and hereby, their genetic diversity and genetic relationship were analyzed. The results show that there are totally 91 bands amplified by ten pairs of SSR primers and percentage of polymorphic band is 100. 00%. The difference of genetic diversity parameters of eight populations is great, and effective number of allele is 1. 437 9-1. 535 0, Nei’s gene diversity index is 0. 256 0-0. 309 2, Shannon information index is 0. 376 7-0. 459 2, percentage of polymorphic band is 64. 84% -85. 71%. The effective number of allele, Nei’s  gene diversity index and Shannon information index among eight populations are 1. 616 9, 0. 355 1 and 0. 528 5, respectively, which is obviously higher than those within population. The gene flow and gene differentiation coefficient of eight populations are 1. 739 5 and 0. 223 3, respectively, indicating that the gene exchange among populations is more. The result of UPGMA cluster analysis shows that eight populations of M. baccata are divided into three groups where Nei’s genetic distance is 0. 148 6. In which, the population in Saihanba of Hebei Province is a group individually, two populations in Mt. Wutai of Shanxi Province and Mt. Dongling of Beijing City are a group, the other five populations are a group. Based on these results, it is presumed that M. baccata is originated from the North and Northeast of China, M. baccata populations in Mt. Lingkong of Shanxi Province, Xiaoxing’anling of Heilongjiang Province, Mt. Changbai of Jilin Province and Mt. Zhongtiao of Shanxi Province may be core populations of genetic diversity.

关键词山荆子; 居群; 遗传多样性; 遗传关系; SSR 标记; 聚类分析
Key wordsMalus baccata ( L.) Borkh.; population; genetic diversity; genetic relationship; SSR marker; cluster analysis
作者王雷宏1, 郑玉红2, 汤庚国3
所在单位1. 安徽农业大学林学与园林学院, 安徽合肥230036; 2. 江苏省•中国科学院植物研究所(南京中山植物园), 江苏南京210014;3. 南京林业大学森林资源与环境学院, 江苏南京210037
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基金项目安徽省自然科学基金资助项目(10040606Q18)