2024年12月17日 星期二
枝菌根真菌对NaCl胁迫下核桃幼苗根系生理及矿质元素吸收的影响
Effects of arbuscular mycorrhizal fungi on root physiology and mineral element uptake of Juglans regia seedlings under NaCl stress
2024年 第33卷 第6期 页码[22-33]    下载全文[1.2MB]  
摘要

 为评估丛枝菌根真菌(AMF)对核桃(Juglans regia Linn.)幼苗耐盐性的促进效果,以核桃品种‘温185’(‘Wen 185’)的盆栽苗为材料,分别接种摩西斗管囊霉〔Funneliformis mosseae (T. H. Nicolson et Gerd.) C. Walker et A. Schüβler〕和异形根孢囊霉〔Rhizophagus irregularis (Blaszk., Wubet, Renker et Buscot) C. Walker et A. Schüβler〕,测定了0、100、200、300 mmol·L-1NaCl处理下根系的生长指标、抗氧化酶活性、渗透调节物质含量、内源激素含量及叶片和根系中矿质元素含量。结果表明:NaCl胁迫下,核桃幼苗的根系生长受到抑制,在300 mmol·L-1NaCl处理下接种2种AMF均显著(P<0.05)提高了根系体积。随着NaCl浓度升高,根系抗氧化酶活性及过氧化氢(H2O2)和丙二醛(MDA)含量整体上显著升高;在300 mmol·L-1NaCl处理下,接种摩西斗管囊霉显著提高了根系超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性,接种异形根孢囊霉显著提高了根系APX活性,接种2种AMF均显著降低了根系H2O2和MDA含量。在300 mmol·L-1NaCl处理下,接种2种AMF均显著提高了根系可溶性糖含量。各浓度NaCl处理下,接种2种AMF均显著提高了根系吲哚乙酸、脱落酸、赤霉素3和玉米素核苷含量。随着NaCl浓度升高,叶片P、K、Ca、Mg含量整体呈上升趋势;在300 mmol·L-1NaCl处理下,接种2种AMF均降低了叶片和根系中P含量,整体显著提高了根系K和Ca含量,接种异形根孢囊霉还显著提高了叶片和根系中Mg含量。在300 mmol·L-1NaCl处理下,接种异形根孢囊霉显著提高了根系Fe含量和叶片Zn含量,接种摩西斗管囊霉显著提高了根系Fe含量。随着NaCl浓度升高,未接菌幼苗叶片和根系中Na含量整体呈上升趋势,而K/Na比、Ca/Na比和Mg/Na比则相反;在300 mmol·L-1NaCl处理下,接种2种AMF均显著降低了根系Na含量,且接种摩西斗管囊霉还显著降低了叶片Na含量并显著提高了叶片和根系中K/Na比、Ca/Na比和Mg/Na比。综上所述,摩西斗管囊霉和异形根孢囊霉对核桃幼苗的耐盐性提高具有明显促进作用,且这2种AMF的作用机制可能存在差异。

Abstract

To evaluate the promoting effects of arbuscular mycorrhizal fungi (AMF) on salt tolerance of Juglans regia Linn. seedlings, the potted seedlings of J. regia  ‘Wen 185’ were taken as materials, the seedlings were inoculated with Funneliformis mosseae (T. H. Nicolson et Gerd.) C. Walker et A. Schüβler and Rhizophagus irregularis (Blaszk., Wubet, Renker et Buscot) C. Walker et A. Schüβler, and the growth indexes, antioxidant enzyme activities, osmotic adjustment substance contents, and endogenous hormone contents in roots, as well as mineral element contents in leaves and roots under 0, 100, 200, and 300 mmol·L-1 NaCl treatments were measured. The results show that under NaCl stress, the root growth of J. regia seedlings is inhibited, and inoculation with  two AMFs both significantly (P<0.05) increase the root volume under 300 mmol·L-1 NaCl treatment. With the increase of NaCl concentration, the antioxidant enzyme activities and contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in roots increase significantly in general; under 300 mmol·L-1 NaCl treatment, inoculation with F. mosseae significantly increases the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in roots, inoculation with R. irregularis significantly increases the APX activity in roots, and inoculation with  two AMFs both significantly decrease the contents of H2O2 and MDA in roots. Under 300 mmol·L-1 NaCl treatment, inoculation with  two AMFs both significantly increase the soluble sugar content in roots. Under  each concentration of NaCl treatment, inoculation with  two AMFs both significantly increase the contents of indoleacetic acid, abscisic acid, gibberellin 3, and zeatin riboside in roots. With the increase of NaCl concentration, the contents of P, K, Ca, and Mg in leaves generally show a tendency to increase; under 300 mmol·L-1 NaCl treatment, inoculation with  two AMFs both decrease the P contents in leaves and roots and significantly increase the contents of K and Ca  in roots in general, and inoculation with R. irregularis also significantly increases the Mg contents in leaves and roots. Under 300 mmol·L-1 NaCl treatment, inoculation with R. irregularis significantly increases the Fe content in roots and Zn content in leaves, while inoculation with F. mosseae significantly increases the Fe content in roots. With the increase of NaCl concentration, the Na contents in leaves and roots of non-inoculation seedlings show a tendency to increase in general, while the ratios of K/Na, Ca/Na, and Mg/Na show the opposite trends; under 300 mmol·L-1 NaCl treatment, inoculation with  two AMFs both significantly decrease the Na content in roots, and inoculation with F. mosseae also significantly decreases the Na content in leaves and significantly increases the ratios of K/Na, Ca/Na, and Mg/Na in leaves and roots. In conclusion, both F. mosseae and R. irregularis have obvious promoting effects on enhancing salt tolerance of J. regia seedlings, and there may be differences in the action mechanisms between the two AMFs.

 

关键词核桃; NaCl胁迫; 丛枝菌根真菌; 根系生理; 矿质元素
Key wordsJuglans regia Linn.; NaCl stress; arbuscular mycorrhizal fungus; root physiology; mineral element
作者李敖, 郑旭, 聂瑞宁, 吴承勖, 唐佳莉, 姬新颖, 张俊佩
所在单位中国林业科学研究院林业研究所 林木遗传育种全国重点实验室 国家林业和草原局林木培育重点实验室, 北京 100091
点击量108
下载次数58
基金项目国家重点研发计划项目(2022YFD2200402)