2021年4月22日 星期四
黄山栾树新品种‘金焰彩栾’叶片呈色的生理特性及影响因子分析
Analyses on physiological characteristics and influence factors of leaf color of new cultivar ‘Jinyan’ of Koelreuteria bipinnata
2020年 第29卷 第6期 页码[51-56]
摘要

以黄山栾树(Koelreuteria bipinnata Franch.)实生苗为对照,对来源于黄山栾树天然黄化突变体的新品种‘金焰彩栾’(‘Jinyan’)在叶片发育期过程中叶色的变化进行了观察,并对其叶色参数(包括L*、a*和b*值)以及叶片的叶绿素a(Chla)、叶绿素b(Chlb)、总叶绿素(Chl)、类胡萝卜素(Car)和花色素苷(Acy)含量及比值,含水量,细胞液pH值和可溶性糖含量的变化进行了测定和分析。结果表明:‘金焰彩栾’叶色呈现“橙红色—黄绿色—金黄色”的变化规律。‘金焰彩栾’叶片的L*值在10月28日最高(82.94),a*值在4月14日最高(56.93),b*值在11月27日最高(46.35),且L*和b*值基本上极显著(P<0.01)高于实生苗。整个实验期间,‘金焰彩栾’叶片的Chla、Chlb、Chl、Car和Acy含量基本上极显著低于实生苗,而Chla/Chlb、Car/Chl和Acy/Chl值基本上显著(P<0.05)或极显著高于实生苗;总体上看,叶片含水量高于实生苗,而细胞液pH值和可溶性糖含量却低于实生苗。多元逐步线性回归分析结果表明:‘金焰彩栾’叶片的a*值与Chl含量显著负相关,L*和b*值与Car含量显著正相关。综上所述,‘金焰彩栾’叶色的适宜观赏期为春季和秋季,其叶色变化是多种因子综合作用的结果,其中,Chl和Car含量及Car/Chl值为主要影响因子。

Abstract

 Taking seedlings of Koelreuteria bipinnata Franch. as the control, the change in leaf color of new cultivar ‘Jinyan’ originated from yellow mutant of K. bipinnata during leaf development process was observed, and the changes in its leaf color parameters (including L*, a*, and b* values), and  contents and ratios of chlorophyll a (Chla), chlorophyll b (Chlb), total chlorophyll (Chl), carotenoid(Car) and anthocyanin (Acy), water content, pH value of cell sap, and soluble sugar content in leaf were assayed and analyzed. The results show that leaf color of ‘Jinyan’ shows a variation law of “orange red-yellow green-golden yellow”. L* value in leaf of ‘Jinyan’ is the highest (82.94) on October 28th, a* value is the highest (56.93) on April 14th, b* value is the highest (46.35) on November 27th, and L* and b* values are extremely significantly (P<0.01) higher than those of seedling in general. During the whole experimental period, Chla, Chlb, Chl, Car, and Acy contents in leaf of ‘Jinyan’ are extremely significantly lower than those of seedling in general, while Chla/Chlb, Car/Chl, and Acy/Chl values are significantly (P<0.05) or extremely significantly higher than those of seedling in general; overall, leaf water content is higher than that of seedling, while pH value of cell sap and soluble sugar content are lower than those of seedling. The multiple stepwise linear regression analysis result shows that a* value in leaf of ‘Jinyan’ is significantly negatively correlated with Chl content, while L* and b* values are significantly positively correlated with Car content. Taken together, the suitable ornamental periods of leaf color of ‘Jinyan’ are spring and autumn, and the change in its leaf color is comprehensive action result of multiple factors, in which, Chl and Car contents and Car/Chl value are the dominant influence factors.

关键词黄山栾树; 黄化突变体; 叶色参数; 色素含量; 生理生化指标; 多元逐步线性回归分析
Key wordsKoelreuteria bipinnata Franch.; yellow mutant; leaf color parameters; pigment content; physiological and biochemical indexes; multiple stepwise linear regression analysis
作者吕运舟1, 董筱昀1, 杨小鑫2, 孙海楠1, 何开跃2, 黄利斌1
所在单位1. 江苏省林业科学研究院, 江苏 南京 211153; 2. 南京林业大学生物与环境学院, 江苏 南京 210037
点击量268
基金项目江苏省自然科学基金青年基金项目(BK20141041)