对改性聚酰胺吸附层析柱进行了系统的研究，利用β 萘酚橙获得了该柱一系列有关信息，如：最佳聚酰胺涂装量，水洗最佳流速范围，最小水洗用量，饱和吸附量。得出了β 萘酚橙在该层析柱上的吸附为单分子层吸附的结论。使用本法在银杏叶黄酮的分离中得到了总黄酮含量超过５０％的满意结果。

The adsorption chromatog raphy column of im proved poly amide is systematically studied .A series of information, such as the best polyamide coat, the best w atering speed zone of the mobile phase, the minimum w atering volume, the maximum adsorptive capacity, are obtained for the column by means of β-naphthol orange method . The conclusion is that the adsorptive type of β-naphthol o range in the column is monomolecular adsorption .This colum n can be used in the ex tract of flavonoids from Gingkgo biloba L .leaves with the satisfied result of over 50 %total flavonoids content .