2024年3月28日 星期四
改性聚酰胺层析柱的性能及其在银杏黄酮类化合物提取中的应用
Properties of the chromatography column of improved polyamide and it′ s application in Gingkgo flavonoids extract
1999年 第8卷 第1期 页码[10-14]    下载全文[0.6MB]  
摘要

对改性聚酰胺吸附层析柱进行了系统的研究,利用β 萘酚橙获得了该柱一系列有关信息,如:最佳聚酰胺涂装量,水洗最佳流速范围,最小水洗用量,饱和吸附量。得出了β 萘酚橙在该层析柱上的吸附为单分子层吸附的结论。使用本法在银杏叶黄酮的分离中得到了总黄酮含量超过50%的满意结果。

Abstract

The adsorption chromatog raphy column of im proved poly amide is systematically studied .A series of information, such as the best polyamide coat, the best w atering speed zone of the mobile phase, the minimum w atering volume, the maximum adsorptive capacity, are obtained for the column by means of β-naphthol orange method . The conclusion is that the adsorptive type of β-naphthol o range in the column is monomolecular adsorption .This colum n can be used in the ex tract of flavonoids from Gingkgo biloba L .leaves with the satisfied result of over 50 %total flavonoids content .
 

关键词改性聚酰胺; 柱层析; β-萘酚橙; 银杏; 黄酮
Key wordsimproved polyamide; colum n chromatography; β-naphthol orange; Gingkgo biloba L .; flavonoids
作者王怡红, 张征林, 宋 苇 ,杨 芝
所在单位东南大学化学化工系, 南京 210096
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