采用离体胚培养法,研究了在培养基中添加 81. 33 mg· L^-1(半抑制浓度,IC₅₀ )香草酸的条件下,青钱柳[Cyclocarya paliurus (Batal.) Iljinsk.]离体胚萌发过程中部分生理生化指标的变化,并对各指标间的相关性进行了分析。结果表明:随培养时间的延长,处理组(培养基中添加 81. 33 mg· L^-1香草酸)及对照组离体胚的粗脂肪和蛋白质含量均呈逐渐降低的趋势,淀粉和可溶性糖含量均呈先降低后升高的趋势,异柠檬酸裂合酶( ICL)、酸性磷酸酶(AP)、碱性磷酸酶(ALP)和过氧化物酶(POD)活性以及葡萄糖-6-磷酸脱氢酶( G-6-PDH)和 6-磷酸葡萄糖酸脱氢酶(6-PGDH)联合活性均高于培养初期,且 ICL、POD、AP 和 ALP 活性不断提高;但处理组的粗脂肪和蛋白质含量均高于对照组且变化幅度相对较小,淀粉含量在培养前期高于对照、在培养后期低于对照,可溶性糖含量总体上高于对照,ICL、AP 和 ALP 活性及 G-6-PDH 和 6-PGDH 联合活性的提高幅度整体上小于对照,培养后期 POD 活性显著高于对照。 相关性分析结果表明:青钱柳离体胚萌发过程中粗脂肪和蛋白质含量与各种酶活性间均呈显著或极显著的负相关。 研究结果显示:香草酸对青钱柳离体胚萌发过程中各种酶的活化产生抑制作用,导致主要贮藏物质的转化受阻,从而影响胚的萌发。

By embryo in vitro culture method, change of some physiological-biochemical indexes and correlation among these indexes during germination process of embryos in vitro of Cyclocarya paliurus (Batal.) Iljinsk. were analyzed under the condition of adding 81. 33 mg · L^-1 ( hemi-inhibitory concentration, IC50) vanillic acid ( VA) in medium. The results show that with prolonging of culture time, contents of crude fat and protein in embryos in vitro of treatment group (adding 81. 33 mg· L^-1 VA in medium) and the control group all appear the trend of decreasing gradually, while contents of starch and soluble sugar all appear the trend of decreasing at first and then increasing, activities of isocitrate lyase (ICL), acid phosphatase (AP), alkaline phosphatase (ALP) and peroxidase (POD), combined activity of glucose-6-phosphate dehydrogenase ( G-6-PDH) and 6-phosphogluconate dehydrogenase (6- PGDH) all are higher than those at start culture stage, and activities of ICL, POD, AP and ALP all increase continuously. But contents of crude fat and protein in embryos in vitro of treatment group are both higher than those of the control group and their change ranges are relatively smaller, starch content is higher at prophase but lower at anaphase than that of the control, soluble sugar content is generally higher than that of the control, enhancement extent of activities of ICL, AP and ALP, combined activity of G-6-PDH and 6-PGDH are generally lower than those of the control, POD activity is significantly higher than that of the control at anaphase. The correlation analysis result shows that there are significant or extremely significant negative correlations among contents of crude fat and protein with activities of above-mentioned enzymes. It is considered that VA has an inhibition to activations of enzymes during germination process of embryos in vitro of C. paliurus and leads to hinder from the transformation of main storage substances, so as to inhibit germination of embryos.