采用水培法研究了Cd-Cu复合胁迫(5 mg•L^-1Cd-10 mg•L^-1Cu;5 mg•L^-1Cd-20 mg•L^-1Cu;25 mg•L^-1Cd-10 mg·L^-1Cu;25 mg•L^-1Cd-20 mg•L^-1Cu)条件下黄菖蒲(Iris pseudacorus L.)叶片和根系对Cd和Cu的积累作用及黄菖蒲体内Cd和Cu迁移率的变化规律。结果表明,在50 d的胁迫期内,黄菖蒲叶片和根系中的Cd和Cu含量分别随培养液中Cd和Cu浓度的提高而增加,并随胁迫处理时间的延长基本呈增加的趋势,在胁迫末期明显提高。在不同浓度Cd-Cu复合胁迫条件下,黄菖蒲根系中的Cd和Cu积累量明显高于叶片。在Cd浓度不变的条件下,黄菖蒲叶片和根系中的Cd含量随培养液中Cu浓度的提高而增加;在Cu浓度不变的条件下,黄菖蒲叶片中Cu含量随培养液中Cd浓度的提高而降低。在不同胁迫时间,黄菖蒲植株对Cd的迁移率不同,但培养液中Cd的浓度较高,黄菖蒲植株对Cd的迁移率也较高;黄菖蒲植株对Cu的迁移率随胁迫时间的延长基本上呈上升趋势。研究结果显示,在Cd-Cu复合胁迫条件下,Cu对黄菖蒲体内Cd的吸收具有一定的协同吸收作用,而Cd对Cu的吸收则有一定的拮抗作用。 

Effects of Cd-Cu combined stress(5 mg•L^-1 Cd-10 mg•L^-1Cu; 5 mg•L^-1 Cd-20mg•L^-1 Cu;  25 mg•L^-1Cd-10 mg•L^-1Cu; 25 mg•L^-1Cd-20 mg•L^-1Cu) on Cd and Cu accumulation in leaf and root of Iris pseudacorus L.and changes of translocation rate of Cd and Cu were  studied by the water culture method. The results show that Cd and Cu contents in leaf and root of I. pseudacorus increase with the increase of Cd and Cu concentrations in medium with stress time prolonging during  50d of stress time, and at the end of stress treatment, the contents of Cd and Cu rise significantly. Accumulation amount of Cd and Cu in root is markedly higher than that in leaf. When Cd concentration remains constant in medium, Cd accumulation in leaf and root of I pseudacorus. increases with Cu concentration in creasing in medium. When Cu concentration remains constant in medium, Cu accumulation in leaf of I. pseudacorus decreases with Cd concentration increasing in medium.Translocation rate of Cd in I. pseudacorus varies at different stress stage, but over all it increases with Cd concentration increasing in medium . Basically, translocation rate of Cu in I. pseudacorus increases with stress time prolonging. It can be concluded that under  Cd-Cu combined stress, Cu has a synergistic effect on Cd accumulation in I. pseudacorus , while Cd has an antagonistic effect on Cu accumulation.