A procedure on cryopreservation of shoot-tips of Eriobotrya japonica Lindl.had been studied in vitro by two steps vitrification. The effects of cold-hardening, preculture, cryoprotecten treatment, thawing and reculture on survival rate of the shoot-tips were tested. Optimal media for shoot-tip induction and propagation were 1/2 MS+1.0 mg·L^-1 6-BA+0.5 mg·L^-1NAA and MS+0.5 mg·L^-16-BA+0.1 mg·L^-1 NAA. Preculture for 30 min with 60% PVS₂(30% glycerol+15% ethylene glycol+15% DMSO+0.4 mol·L^-1 sucrose) and treatment for 50 min by PVS₂ were suitable to shoot-tip cryopreservation. After cryopreservation some shoot-tips could regenerate plantlets, the regenerate rate was 46.8%.There was no difference in regenerating plantlets and tube plantlets not stored by cryopreservation.