以人参(Panax ginseng C. A. Mey.)培养细胞为生物反应体系,利用外源氢醌为底物,对熊果苷的生物合成进行了研究。TLC鉴别表明,人参细胞可以将外源的氢醌转化为熊果苷;以熊果苷含量和氢醌的转化率为指标,对人参细胞生物合成熊果苷的基本条件(氢醌浓度、转化持续时间、细胞培养阶段)进行了探讨,结果表明,MS固体培养基上培养32d的人参细胞,在含有2mmol·L-1氢醌的生物合成培养基中转化24h后,合成的熊果苷含量占细胞干重的7.176%,氢醌转化率也达到79.15%。

Using hydroquinone as a substrate, the arbutin biosynthesis was investigated by Panax ginseng C. A. Mey. cell suspension culture. The result of identification of TLC indicated that P. ginseng cells were able to convert hydroquinone into arbutin. Based on the content of arbutin and the transformation rate of hydroquinone, these basic conditions(such as hydroquinone concentration, lasting time and the culture stage) were studied for biosynthesis arbutin by P. ginseng cell. The results showed that the content of arbutin and the transformation rate of hydroquinone were 7.176% and 79.15% respectively when P. ginseng cells cultured in MS medium for 32 d were transferred in biotransformation medium containing 2 mmol·L^-1hydroquinone and incubated 24 h.