2024年3月29日 星期五
基于SRAP 标记的墨西哥落羽杉优良单株的遗传多样性分析
Genetic diversity analysis of superior individuals of Taxodium mucronatum based on SRAP marker
2012年 第21卷 第1期 页码[36-41]    下载全文[0.7MB]  
摘要

采用SRAP 标记方法,分析了原产地及种植地不同的18 个墨西哥落羽杉(Taxodium mucronatum Tenore)优良单株的遗传多样性,并根据遗传相似系数、采用UPGMA 法对它们的遗传关系进行了聚类分析。结果显示:用7 对引物组合从18 个单株的总DNA 中共扩增出87 条带,其中多态性条带71 条,多态性条带百分率为81. 6%。按照原产地和种植地可将18 个单株分为4 组,它们的总体Nei’s 基因多样性指数、Shannon 信息指数和基因分化系数分别为0. 229、0. 355 和0. 479 9,而基因流仅为0. 542,说明各组间的基因交流较少。18 个单株间的遗传相似系数为0. 632 2 ~0. 919 5,平均值为0. 753 9。聚类分析结果显示:18 个单株可分为3 组,其中18 号和14 号单株分别单独成组,其余16 个单株聚为1 组。后者可进一步分为8 个亚组:1 号、7 号、12 号和15 号单株各自单独成亚组; 2 号、3 号、5 号、9 号、11 号和13 号单株聚为1 个亚组;4 号和6 号单株、8 号和17 号单株、10 号和16 号单株分别聚为3个亚组。研究结果表明:18 个优良单株间存在丰富的遗传变异,但它们的遗传关系与原产地及种植地明显不相关。

Abstract

Genetic diversity of eighteen superior individuals of Taxodium mucronatum Tenore from different origins and cultivating places was analyzed by SRAP marker method, and the genetic relationship among eighteen individuals was also analyzed by using UPGMA method based on genetic similarity coefficient. The results show that 87 bands are amplified from total DNA of eighteen superior individuals by seven pairs of primer combinations, in which, there are 71 polymorphic bands with the polymorphic band percentage of 81. 6%. Eighteen individuals are divided into four groups according to origin and cultivating place, their total Nei’s gene diversity index, Shannon information index and genetic differentiation coefficient are 0. 229, 0. 355 and 0. 479 9, respectively, but their gene flow is only 0. 542, which shows that there is a little gene intercommunion among different groups. Genetic similarity coefficient among eighteen individuals is 0. 632 2-0. 919 5 with an average value of 0. 753 9. The result of cluster analysis shows that eighteen superior individuals are divided into three groups, in which, individuals of No. 18 and No. 14 are independent respectively, and other sixteen individuals form. a group. The latter is further divided into eight sub-groups, in which, individuals of No. 1, No. 7, No. 12 and No. 15 are respectively independent, individuals of No. 2, No. 3, No. 5, No. 9, No. 11 and No. 13 form. a sub-group, and every two individuals of No. 4 and No. 6, No. 8 and No. 17, No. 10 and No. 16 form. three sub-groups, respectively. It is suggested that there is rich genetic diversity among eighteen superior individuals, while their genetic relationship is not obviously related to origin and cultivating place.

 

关键词墨西哥落羽杉; 优良单株; SRAP 标记; 遗传多样性; 聚类分析
Key wordsTaxodium mucronatum Tenore; superior individual; SRAP marker; genetic diversity; cluster analysis
作者周冬琴, 莫海波, 芦治国, 於朝广, 徐和宝, 殷云龙
所在单位江苏省•中国科学院植物研究所(南京中山植物园), 江苏南京21001
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基金项目江苏省林业三项工程项目[lysx(2007)02]