2024年5月22日 星期三
人参毛状根生物合成天麻素转化体系的建立
Establishment of biotransformation system of the gastrodin biosynthesis by hairy root of Panax ginseng
2005年 第14卷 第2期 页码[29-31]    下载全文[0.4MB]  
摘要

以对羟基苯甲醇转化率和天麻素含量为指标,探索适宜于人参毛状根生物合成天麻素的培养条件如底物浓度、转化持续时间和培养阶段。结果表明,B5液体培养基培养22d的人参毛状根,在含有1.000mmol·L-1对羟基苯甲醇的生物合成培养基中转化24h,合成的天麻素含量占干重的6.65%,对羟基苯甲醇转化率达到84.8%,说明以人参毛状根为反应器可以合成人参所不具有的天麻素。

Abstract

Using the content of gastrodin and the transformation rate of exogenous 4-hydroxybenzyl alcohol as indexes, it was carried on to research the proper biosynthesis conditions such as the substrate concentration, transformative lasting time and the culture stage of hairy root of Panax ginseng C.A.Mey. The results indicated that the content of gastrodin and the transformation rate of exogenous 4-hydroxybenzyl alcohol were 6.65% and 84.5% respectively when the hairy root of P. ginseng cultured in B5 liquid medium for 22d, were transformed in biotransformation medium containing 1. 000 mmol·L-1 4-hydroxybenzyl alcohol and incubated 24 h. It showed that gastrodin is synthesized by the hairy root of P. ginseng.

关键词人参; 毛状根; 生物合成; 天麻素; 对羟基苯甲醇;
Key wordsPanax ginseng C.A.Mey.; hairy root; biosynthesis; gastrodin; 4-hydroxybenzyl alcohol
作者蔡洁,丁家宜,华亚男,李楠
所在单位中国药科大学中药生物技术教研室,江苏南京210038
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