2024年12月17日 星期二
美味猕猴桃品种‘金魁’离体叶片不定芽诱导及生根培养基筛选
Screening on induction and rooting media of adventitous buds from in vitro leaf of cultivar ‘ Jinkui’of Actinidia chinensis var. Deliciosa
2016年 第25卷 第1期 页码[105-107] 下载全文[1.4MB]
| Abstract | In order to establish high frequency regeneration system of cultivar ‘Jinkui’of Actinidia chinensis var. deliciosa (A. Chev.) A. Chev., the optimum media for induction and rooting of adventitious buds were screened by taking in vitroleaf of sterile seedling as explant. The results show that the optimum medium for induction of adventitious buds of ‘Jinkui’ is MS medium containing 3. 0 mg·L-1 6-BA and 0. 2 mg·L-1 NAA with regeneration rate of 94. 4%. The optimum medium for rooting of adventitious buds is 1/2MS medium containing 0. 7 mg·L-1 IBA with rooting rate of 100. 0%. |
| 关键词 | 美味猕猴桃; 离体叶片; 诱导培养基; 生根培养基 |
| Key words | Actinidia chinensis var. deliciosa (A. Chev.) A. Chev.; in vitro leaf; induction medium; rooting medium |
| 作者 | 黄胜男1, 张计育1, 毛妮妮2, 王刚1, 宣继萍1, 贾晓东1, 翟敏1, 郭忠仁1 |
| 所在单位 | 1. 江苏省中国科学院植物研究所(南京中山植物园), 江苏南京210014; 2. 江苏丘陵地区镇江农业科学研究所, 江苏镇江212400 |
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| 基金项目 | 国家自然科学基金资助项目(31401854); 江苏省自然科学基金资助项目(BK20140760); 江苏省现代农业———研究开发示范类项目(BE2015350); 泰州市科技支撑计划(农业)项目(TN201511) |