2024年12月22日 星期日
菠萝各器官蛋白酶的提取与保活
The extraction and protection of enzyme activity of bromelin from organs of pineapple
1994年 第3卷 第2期 页码[22-26]    下载全文[0.7MB]  
摘要

研究了菠萝各器官的蛋白酶含量、活性和提取过程中酶活性的保护作用,结果表明,无刺卡因品种的青果、70%成熟果肉、70%成熟果皮、茎、果柄、叶的粗酶含量分别为0.359、0.194、0.132、0.994、0.303和0.195%。各器官酶活性以青果酶活性最高。在提取过程中使用乙二胺四乙酸二钠、醋酸锌、氯化钠、抗坏血酸可使果肉蛋白酶、茎蛋白酶和叶蛋白酶活性分别提高69.37%、93.88%和139.69%。1000ppm硫代硫酸钠+1000ppm半胱氨酸是菠萝蛋白酶活性的有效保护剂,可使果蛋白酶和茎蛋白酶活性分别提高35.51%和69.06%。

Abstract

The contents and the activities of bromelin in organs of pineapple, and the protection of enzyme activities during extracting process were investigated. It was indicated that the contents of bromelin in green fruit, fruit pulp and skin of 70% ripeness, stem, stalk and leaf of "Wucikayin" cultivar were 0. 359, 0. 194, 0. 132, 0. 994, 0. 303, and 0. 195% respectively. The activity of bromelin in green fruit was the highest among all those of organs. The use of disodiurn EDTA, zinc acetate, sodium chloride and ascorbic acid in the extracting process could raise the activities of brornelin in fruit pulp , stem and leaf by 69. 37%, 93. 88% and 139. 69% respectively. It was discovered that 1 000 ppm of sodium hyposulfate plus 1 000 ppm of cysteine was an effective protector of enzyme activity and raised the activities of fruit bromelin and stem bromelin by 35. 51% and 69. 06%.

关键词菠萝蛋白酶; 提取; 活性保护剂; 硫代硫酸钠; 半胱氨酸;
Key wordsbromelin; extraction; activity protector; sodium hyposulfate; cysteine
作者林韶湘, 黄卓烈 ,李明启, 陈永泉
所在单位华南农 业 大学生物系 . 广 州 510642
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