以‘津研4号’黄瓜(Cucumis sativus ‘Jinyan No.4’)为接穗,云南黑籽南瓜(Cucurbita ficifolia Bouché)和黄瓜为砧木,研究了低温条件下黄瓜/云南黑籽南瓜嫁接株(黄瓜/南瓜)、黄瓜/黄瓜嫁接株和自根黄瓜株叶片的蔗糖(Suc)、葡萄糖(Glc)和可溶性蛋白质(Pr)含量的变化以及叶片羧化效率(CE)及不同叶位叶片、不同节位茎段中异戊烯基腺嘌呤核苷(iPA)含量的差异。结果表明,黄瓜/南瓜嫁接株叶片的Suc、Glc及Pr含量和CE值均显著高于自根黄瓜株和黄瓜/黄瓜嫁接株,自根黄瓜株与黄瓜/黄瓜嫁接株间差异不显著。iPA在嫁接植株和自根植株茎中均呈梯度分布,其含量在生长锥中最高,其次为上部茎段,下部茎段中的含量最低;自根黄瓜株和黄瓜/黄瓜嫁接株茎中的iPA含量显著低于黄瓜/南瓜嫁接株,而叶片中iPA含量显著高于黄瓜/南瓜嫁接株。研究结果表明,以黑籽南瓜为砧木可提高嫁接黄瓜对低温的耐受性。

Using cucumber(Cucumis sativus`JinyanNo. 4')as ingrafment and cucumber and fig leaf gourd (Cucurbita ficifolia Bouché)as stocks, the tolerance of own-rooted and grafted cucumbers to low temperature was evaluated. Contents of sucrose (Suc), glucose (Glc) and soluble protein (Pr) in leaf and content of isopentenyl adenosin (iPA) in stem and leaf were determined respectively, and carboxylation efficiency(CE) was calculated. The results showed that Suc,  Glc,  Prand CE in cucumber/fig leaf gourd grafted plant were higher than those in own-rooted and cucumber/cucumber grafted plants. The iPA distributed with a gradient in stem of both grafted and own-rooted plants, iPA content was the highest in stem apex, followed by upper stem segments, and then lower stem segments. The iPA content in stem of both own-rooted and cucumber/cucumber grafted plants was less than that of cucumber/fig leaf gourd grafted plant, while iPA  content in leaves of both own-rooted and cucumber/cucumber grafted plants was higher than that of cucumber/fig leaf gourd grafted plant. It is suggested that the tolerance of grafted cucumber to low temperature is enhanced while fig leaf gourd as stock is used.