用台湾金线兰 ( Anoectochilus formosanus Hay. )的茎段作外植体 ,比较不同的培养基、蔗糖浓度、有机添加物、 p H值、激素等因素对丛生芽生成的影响 ,实验证明 B5及 M S较好 ,蔗糖浓度为2% ～ 3% ,在细胞分裂素试验中发现 BA作用优于 KT和 ZT,最适浓度为 4 mg /l,还证明 4Pu-30在 0. 5 mg /l浓度下即有很好的作用。 台湾金线兰组织培养苗及与同属植物花叶开唇兰 ( A. rox -burghii ( W all. ) Lindl. )组培苗和野生药材的氨基酸、维生素的含量测定 ,结果表明台湾金线兰的氨基酸含量较高 ,并有丰富的 VC, VB1, VB2, VE, 尼克酸及胡罗卜素 ,对进一步直接开发利用金线连组织培养苗提供了依据。
 

The cluster shoots w ere obtained by stem segment of A. formosanus in tissue culture. Comparison of different m edia, sucrose concentration, p H value, organic extract and hormone were investigated.  It was found that B5 and MS medium with 2%～ 3% surose, 4 mg /lBA or 0. 5 mg /l 4Pu-30 show ed the ideal result. Compared with cultured seedling and crude drug of wild species ( A. roxburghii) , A. formosanus seedling has high content of amino acids and rich in VC, VB1, VB2 , VE, nicotinic acid and carotene. It will provide the basis for further development and utilization of A. formosanus from tissue culture.