2021年10月27日 星期三
4种吴茱萸属植物叶片和果实的乙醇提取物及其体外抗肿瘤活性
Ethanol extracts from leaves and fruits of four Evodia species and their in vitro antitumor activities
2021年 第30卷 第2期 页码[77-79]    下载全文[0.7MB]  
Abstract

Total extracts of 80% (volume fraction) ethanol (hereinafter referred to as total extracts) and its dichloromethane, ethyl acetate, and water fractions were obtained from leaves and fruits of four Evodia J. R. et G. Forst. species by using reflux extraction and solvent extraction, and their in vitro antitumor activities and toxicities were evaluated by using MTT method. The results show that the extraction rates of total extracts from leaves and fruits of E. ailanthifolia Pierre, E. austrosinensis Hand.-Mazz., E. daniellii (Benn.) Hemsl., and E. delavayi Dode are in the range of 13.00%-30.35%. The pharmacological experiment result shows that at the drug concentration of 10 μg·mL-1, the inhibition rates of total extracts and its water fraction from leaves of E. ailanthifolia, ethyl acetate fraction of total extracts from fruits of E. austrosinensis, and total extracts from fruits of E. daniellii on breast cancer cell MDA-MB-231 and hepatoma cell HepG2 are higher than 50%, those of dichloromethane fraction of total extracts from leaves of E. ailanthifolia on three tumor cells are all higher than 50%, those of ethyl acetate fraction of total extracts from leaves of E. ailanthifolia on breast cancer cell MDA-MB-231 and colon cancer cell LoVo are higher than 50%, and their inhibition rates on the normal human aortic smooth muscle cell HASMC are lower than 40%. Therefore, above extracts have potential values as a source of natural antitumor drug.

关键词吴茱萸属; 抗肿瘤活性; MTT法; 毒性评价
Key wordsEvodia J. R. et G. Forst.; antitumor activity; MTT method; toxicity evaluation
作者靳桐a, 印敏a, 管福琴a, 李冬玲b, 佟海英b, 冯煦a, 单宇a,①, 王奇志a,
所在单位江苏省中国科学院植物研究所(南京中山植物园): a. 江苏省植物资源研究与利用重点实验室, b. 江苏省农业种质资源保护与利用平台, 江苏 南京210014
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基金项目江苏省林业科技创新与推广项目(LYKJ[2020]09); 江苏省植物资源研究与利用重点实验室开放基金项目(JSPKLB201925; JSPKLB202051)