摘要 | 研究了NaCl胁迫对南瓜 (Cucurbita moschata Duch. )、笋瓜 (C. maxima Duch. )和西葫芦 (C. pepo L. )种子萌发的影响及不同浓度外源Ca2+和GA3对NaCl胁迫下南瓜种子发芽的效应。结果表明,用 30mmol·L-1 NaCl处理,南瓜种子的发芽率高于对照(蒸馏水),而用 100和 170mmol·L-1 NaCl处理,西葫芦和笋瓜种子的发芽率下降率为负值,表明较低浓度NaCl胁迫可一定程度提高西葫芦、笋瓜和南瓜种子的发芽率;高浓度NaCl胁迫对种子发芽有明显的抑制作用。种子萌发期耐盐能力从大至小依次为西葫芦、南瓜、笋瓜。在 170mmol·L-1 NaCl胁迫下,施加 5~30mmol·L-1外源Ca2+或浸种处理,对南瓜种子发芽有促进作用,但高浓度外源Ca2+ (≥50mmol·L-1CaCl2 )则具有抑制作用。在 170mmol·L-1 NaCl胁迫下,用不同浓度GA3浸种处理,对南瓜种子发芽有抑制作用。 |
Abstract | The effect of NaCl stress on seed germination of Cucurbita moschata Duch., C. maxima Duch., C. pepo L. and the effects of exogenous Ca2+and GA3 on seed germination of C. moschata under NaCl stress were studied. The results showed that the germination percentage of C. moschata seed under 30mmol·L-1 NaCl stress was higher than that of the control. When the concentration of NaCl was up to 100 mmol·L-1 or 170mmol·L-1, the decline percentages of germination percentages of C. maxima and C. pepo were negative, it indicated that the seed germination percentages of the three vegetables were increase in some degree by stressing with lower concentration of NaCl. And NaCl stress at higher concentration inhibited obviously the seed germination of these species. The order from high to low of the tolerance to NaCl was C. pepo, C. moschata, C. maxima during germination period. Treating the seeds of C. moschata with 5-30mmol·L-1 CaCl2 or adding exogenous Ca2+ could improve the germination of the seed stressed with 170mmol·L-1NaCl, but it could be inhibited by adding exogenous Ca2+ at high concentration(≥ 50mmol·L-1CaCl2). Dipping the C. moschata seeds in the different concentrations of GA3 solutions could inhibite the germination of the seed stressed with 170 mmol·L-1NaCl. |
关键词 | 南瓜属; NaCl胁迫; 外源钙; GA3; 种子发芽 |
Key words | Cucurbita L.; NaCl stress; exogenous Ca2+; GA3; seed germination |
作者 | 王广印1,韩世栋2,赵一鹏1,周秀梅1,李新峥1 |
所在单位 | 1.河南科技学院,河南新乡453003; 2.潍坊职业学院,山东潍坊261041 |
点击量 | 1434 |
下载次数 | 1071 |
基金项目 | 河南省科技攻关项目(0224310025和 0424040014); |