Taking ripe fruits of 4 Prunus salicina Lindl. cultivars （lines） of ‘Huangguan’， ‘Blackamber’， ‘Elodrao’ and ‘Xigua’ as research materials， contents of organic acids and V_C were determined by UPLC method. The results show that contents of organic acids and VC in fruit of P. salicina cultivars （lines） are evidently different， in which， content of malic acid is the highest （1.172 0-1.991 1 mg·g^-1） among organic acid components， followed by that of tartaric acid （0.063 8-0.418 9 mg·g^-1）， while contents of citric acid， succinic acid， oxalic acid and acetic acid are all lower than or equal to 0.071 5 mg·g^-1， and content of fumaric acid is trace； total content of organic acids and content of VC are 1.356 2-2.162 0 and 0.017 0-0.022 8 mg·g^-1， respectively. Among 4 cultivars （lines）， contents of each organic acid component and VC and total content of organic acids in fruit of ‘Huangguan’ are relatively balanced in general.