摘要 | 刺槐( Robinia pseudoacacia Linn.) 、湿地松( Pinus elliottii Engelm .) 和侧柏〔Platycladus orientalis ( L.) Franco〕种子经 0.3% CaCl2, 250 μg/g H3BO3和 250 μg/g 多效唑( M ET ) 浸种 24 h,然后测定幼苗的耐盐性。 结果表明, 在 0 .3 % NaCl 胁迫条件下, 不同种子预处理方法对种子发芽率、平均苗高、根茎比、简易活力指数( SV IS) 以及幼苗中 Na +与 K +的累积量、 Na +/ K +比及游离脯氨酸含量有较大影响。 树种不同, 处理效果也存在明显差异。 湿地松以 0.3% CaCl2, 侧柏以 250μg/ g H3BO 3浸种处理效果最好, 显著提高种子发芽率、平均苗高、根茎比和 SV IS, 降低幼苗 Na +吸收量和 Na +/K +比, 缓解盐胁迫逆境。 3 种种子预处理方法均降低刺槐种子发芽率、平均苗长和SV IS, 但提高刺槐幼苗游离脯氨酸含量和幼苗根茎比, 减少幼苗对 Na +的吸收, 降低 Na +/K +比。 |
Abstract | The effects of seed pretreatment methods on improving salt tolerance of the seedlings of 3 tree species were studied after soaking seeds of Robinia pseudoacacia Linn ., Pinus elliottii Engelm. and Platycladus orientalis ( L .) Franco with 0 .3 %CaCl2, 250 μg/g H3BO3 and 250 μg/g multi-effect triazole ( MET) fo r 24 h respectively .The results indicated that various seed pretreatment methods had a great influence on seed germination percentage, average seedling height, root/stem ratio, seed vigour index ( SVIS) , the contents of Na+, K+ and free proline in seedlings, and Na +/K + ratio under the 0 .3 % NaCl stress .How ever, for various tree species, the influence was different .Soaking seeds of P. elliottii with 0 .3 % CaCl2 and soaking seeds of P . |
作者 | 方升佐, 曹福亮, 戴蒲英 |
所在单位 | 南京林业大学森林资源与环境学院, 南京 210037 |
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