以日温25 ℃、夜温20 ℃为对照，对高温（日温35 ℃、夜温30 ℃）胁迫32 d内野扇花（Sarcococca ruscifolia Stapf）植株生长及叶中生理生化指标和极性代谢产物含量的变化进行了比较。结果表明：高温胁迫下野扇花大部分叶片明显干枯、变黄，甚至萎蔫、脱落。总体来看，随着高温胁迫时间延长，野扇花叶中叶绿素和类胡萝卜素含量逐渐下降；丙二醛（MDA）含量逐渐升高；电解质外渗率在处理8 d低于0 d，之后逐渐升高，并在处理32 d显著（P＜0.05）升高；超氧化物歧化酶（SOD）活性不断升高；过氧化物酶（POD）活性先下降后升高，并在处理24 d最低；抗坏血酸过氧化物酶（APX）活性先升高后降低并趋于平稳。高温胁迫下，野扇花叶中叶绿素和类胡萝卜素含量及POD活性总体上低于对照，而MDA含量以及SOD和APX活性一直高于对照；电解质外渗率则表现为在处理8、16和24 d显著低于对照，但在处理32 d显著高于对照。依据变量投影重要性值大于1且在高温胁迫和对照间差异显著的标准，从野扇花叶中筛选出22种差异极性代谢产物，包括4种有机酸类成分、7种氨基酸类成分、10种糖和糖醇类成分及1种不饱和脂肪酸类成分，这些差异极性代谢产物可能影响野扇花对高温胁迫的抗性。综上所述，野扇花对高温胁迫较为敏感，且长时间的高温胁迫对野扇花叶中差异极性代谢产物积累的影响更大。

Taking day temperature of 25 ℃ and night temperature of 20 ℃ as the control， plant growth and changes of physiological and biochemical indexes and polar metabolite contents in leaves of Sarcococca ruscifolia Stapf during high temperature （day temperature of 35 ℃ and night temperature of 30 ℃） stress for 32 d were compared. The results show that under high temperature stress， most leaves of S. ruscifolia evidently wither and turn yellow， even wilt and fall. Overall， with the elongation of high temperature stress time， contents of chlorophyll and carotenoid in leaves of S. ruscifolia decrease gradually； malondialdehyde （MDA） content increases gradually； electrolyte leakage at treatment for 8 d is lower than that at treatment for 0 d， then increases gradually， and has a significant （P＜0.05） increase at treatment for 32 d； superoxide dismutase （SOD） activity increases continuously； peroxidase （POD） activity first decreases and then increases， and is the lowest at treatment for 24 d； ascorbate peroxidase （APX） activity first increases and then decreases and finally tends to be steady. Under high temperature stress， contents of chlorophyll and carotenoid and POD activity of leaves of S. ruscifolia are lower than those of the control in general， while MDA content and SOD and APX activities are always higher than those of the control； electrolyte leakage is significantly lower than that of the control at treatment for 8， 16， and 24 d， but is significantly higher than that of the control at treatment for 32 d. 22 differential polar metabolites are screened from leaves of S. ruscifolia according to the criteria of variable importance in projection greater than 1 and significant difference between the high temperature stress and the control， including 4 organic acids， 7 amino acids， 10 saccharides and saccharide alcohols， and 1 unsaturated fatty acid, these differential polar metabolites may affect the resistance of S. ruscifolia against high temperature stress. In conclusion， S. ruscifolia is sensitive to high temperature stress， and the effect of high temperature stress for long time on accumulation of differential polar metabolites in leaves of S. ruscifolia is greater.