通过比较3种大孔吸附树脂对海边月见草(Oenothera littaralis Schlect.)总黄酮的吸附能力,选择了吸附量较大且易洗脱的树脂AB-8,研究了提取液浓度、pH值对该树脂静态吸附能力的影响,以及洗脱剂种类、乙醇浓度对动态解吸能力的影响。结果表明,AB-8树脂对海边月见草总黄酮有良好的吸附纯化性能,当原液浓度为1.076mg·mL^-1时,树脂达饱和吸附量36.11 mg·g^-1;提取液pH值对该树脂的吸附能力影响显著,pH值达4.0~4.5时树脂吸附量最大;用60%乙醇为洗脱剂,流速为1 mL·min^-1,总黄酮的动态洗脱率达83.41%,获得的总黄酮纯度为24.13%,得率3.54%;而30%乙醇(6倍柱床体积)和50%乙醇(6倍柱床体积)组合是最佳动态洗脱剂。

AB-8 resin, which had higher adsorption capacity and easier desorption, was selected from three kinds of macroporous resins. Effects of concentration of extract solution and pH value on static adsorption capacity, and effects of elution agent type and ethanol concentration on dynamic elution were studied. The results showed thatAB-8 resin was suitable for adsorbing and purifying total flavonoids from Oenothera littaralis Schlect. leaves. The maximum static adsorption capacity of AB-8 resin for the flavonoids was up to 36. 11 mg·g^-1 when the concentration of extract solution was 1. 076 mg·mL^-1.The pH value of extract solution had significantly effect on resin absorption properties, the adsorption capacity reached the highest when pH value was pH 4. 0 -pH 4. 5. When the eluant was 60% ethanol at flow velocity 1 mL·min^-1, the dynamic elution ratio of total flavonoids was 83. 41%, the purity of flavonoids obtained was 24. 13% and yield 3. 54% as well. The optimal eluting solvent was the water solutions of 30% ethanol (6 times of resin volume) and 50%  ethanol (6 times of resin volume).