摘要 | 采用超高效液相色谱-电喷雾-串联质谱(UPLC-ESI-MS/MS)技术对黑豆种皮体积分数70%乙醇提取物中的化合物进行分析。结果表明:黑豆种皮体积分数70%乙醇提取物中32个化合物主要为多酚类,其中,酚酸类化合物的相对含量为13.38%,黄酮类化合物的相对含量为39.59%。儿茶素的相对含量最高(10.15%),反式阿魏酸的相对含量次之(7.86%),二氢黄豆苷元和原花青素B1的相对含量也较高(分别为5.52%和5.36%)。黑豆种皮中主要的多酚类物质为酚酸类和黄酮类化合物,具有进一步开发利用的价值。 |
Abstract | The compounds in the volume fraction of 70% ethanol extracts from seed coat of black soybean were analyzed by using ultra-high performance liquid chromatography-electrospray-tandem mass spectrometry (UPLC-ESI-MS/MS) technology. The results show that 32 compounds in the volume fraction of 70% ethanol extracts from seed coat of black soybean are mainly polyphenols, in which, the relative content of phenolic acids is 13.38%, and that of flavonoids is 39.59%. The relative content of catechin is the highest (10.15%), followed by trans-ferulic acid (7.86%), and the contents of dihydrodaidzein and procyanidin B1 are also relatively high (which are 5.52% and 5.36% respectively). The main polyphenols in seed coat of black soybean are phenolic acids and flavonoids, which have valuable potential for further development and utilization. |
关键词 | 黑豆种皮; 化学成分; UPLC-ESI-MS/MS |
Key words | seed coat of black soybean; chemical component; UPLC-ESI-MS/MS |
作者 | 吴志华1,2, 马丽2, 陈剑2, 周佳宇2 |
所在单位 | 1. 南京中医药大学, 江苏 南京 210023; 2. 江苏省中国科学院植物研究所(南京中山植物园)江苏省植物资源研究与利用重点实验室 江苏省抗糖尿病药物筛选技术服务中心, 江苏 南京 210014 |
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基金项目 | 江苏省植物资源研究与利用重点实验室开放基金项目(JSPKLB202201) |