The contents of syringaresnol diglucoside and geniposidic acid in spring leaves and autumn leaves of Eucommia ulmoides Oliv. in 17 provenances were determined by HPLC. The contents of syringaresnol diglucoside in spring leaves and autumn leaves were 0.147%-0.023% and 0.314%-0.011% respectively. The contents of geniposidic acid in spring leaves was not detectable and in autumn leaves 3.98%-0.37%. The contents of syringaresnol diglucoside and geniposidic acid in autumn leaves were much higher than that in spring leaves. The contents of two components in 17 provenances were also distinctive different.