2024年7月26日 星期五
肾叶天胡荽精油的超临界CO2萃取、成分分析及杀虫活性初探
Supercritical CO2 extraction and component analysis of essential oil from Hydrocotyle wilfordi and preliminary study of its insecticidal activity
2008年 第17卷 第4期 页码[27-32]    下载全文[0.6MB]  
摘要

采用正交实验设计,对肾叶天胡荽(Hydrocotyle wilfordi Maxim.)精油超临界CO2萃取条件进行了优化,并用GC-MS分析技术对精油的化学成分进行了鉴定,初步探讨了肾叶天胡荽精油对小菜蛾2龄幼虫的触杀活性。结果表明,肾叶天胡荽精油超临界CO2萃取的最佳条件为萃取压力35 MPa、萃取温度50℃、解析压力5 MPa、解析温度40℃、萃取时间75 min、CO2流量34~36 L·h-1,精油得率最高可达5.32%。从精油中共鉴定出19种成分,总相对含量达到89.266%,主要成分为邻苯二甲酸二辛酯(64.302%)。用超临界CO2萃取技术提取的肾叶天胡荽精油对小菜蛾2龄幼虫有一定的触杀作用,20 g·L-1精油对小菜蛾2龄幼虫的24 h校正死亡率高达90.00%。

Abstract

The optimal conditions of supercritical CO2 extraction for essential oil from Hydrocotyle wilfordi Maxim. were selected by orthogonal test design, and the chemical components in the essential oil were Identified by GC-MS. The contact toxicity of the essential oil against the 2nd in star larvae of Plutella xylostella L. was studied preliminarily.The optimal conditions of supercritical CO2 extraction are as follows: extraction pressure 35 MPa, extraction temperature 50 ℃ , separation pressure 5 MPa, separation temperature 40℃ ,extraction time 75min and CO2 flow 34- 36L·h- 1. And the highest extraction rate of the essential oil reaches 5.32%. Nineteen components in the essential oil have been identified, which account for 89. 266% of the total relative content of the essential oil. The major component is bis(2-ethylhexyl)-phthalate(64.302%). The essential oil of H. wilfordi has a certain contact toxicity against the 2nd instar larvae of P. xylostella, and the corrected mortality of the larvae treated with 20g·L- 1 essential oil for 24h reaches 90.00%.

关键词肾叶天胡荽; 精油; 超临界CO2萃取技术; 气相色谱-质谱分析; 杀虫活性
Key wordsHydrocotyle wilfordi Maxim.; essential oil; supercritical CO2 extraction technology; GC-MS; insecticidal activity
作者胡艳莲,叶 舟,陈 伟,龙 川,李会霞
所在单位福建农林大学生命科学学院,福建福州350002
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基金项目国家林业局林业新品种新技术中间试验计划项目〔(2006)29号〕; 福建省科技计划项目(2001I033);