采用正交实验设计,对肾叶天胡荽(Hydrocotyle wilfordi Maxim.)精油超临界CO₂萃取条件进行了优化,并用GC-MS分析技术对精油的化学成分进行了鉴定,初步探讨了肾叶天胡荽精油对小菜蛾2龄幼虫的触杀活性。结果表明,肾叶天胡荽精油超临界CO₂萃取的最佳条件为萃取压力35 MPa、萃取温度50℃、解析压力5 MPa、解析温度40℃、萃取时间75 min、CO₂流量34～36 L·h^-1,精油得率最高可达5.32%。从精油中共鉴定出19种成分,总相对含量达到89.266%,主要成分为邻苯二甲酸二辛酯(64.302%)。用超临界CO₂萃取技术提取的肾叶天胡荽精油对小菜蛾2龄幼虫有一定的触杀作用,20 g·L^-1精油对小菜蛾2龄幼虫的24 h校正死亡率高达90.00%。

The optimal conditions of supercritical CO₂ extraction for essential oil from Hydrocotyle wilfordi Maxim. were selected by orthogonal test design, and the chemical components in the essential oil were Identified by GC-MS. The contact toxicity of the essential oil against the 2nd in star larvae of Plutella xylostella L. was studied preliminarily.The optimal conditions of supercritical CO2 extraction are as follows: extraction pressure 35 MPa, extraction temperature 50 ℃ , separation pressure 5 MPa, separation temperature 40℃ ,extraction time 75min and CO2 flow 34- 36L·h- 1. And the highest extraction rate of the essential oil reaches 5.32%. Nineteen components in the essential oil have been identified, which account for 89. 266% of the total relative content of the essential oil. The major component is bis(2-ethylhexyl)-phthalate(64.302%). The essential oil of H. wilfordi has a certain contact toxicity against the 2nd instar larvae of P. xylostella, and the corrected mortality of the larvae treated with 20g·L^{- 1} essential oil for 24h reaches 90.00%.