2019年10月17日 星期四
外植体类型和培养基中激素浓度对香茶菜愈伤组织诱导的影响
Effects of explant type and hormone concentration in medium on callus induction of Isodon amethystoides
2017年 第26卷 第1期 页码[113-115]    下载全文[1.2MB]  
Abstract

Effects of explant type (leaf and stem segment) and hormone concentration (0.1, 0.2 and 0.3 mg·L-1 6-BA; 1.0, 1.5 and 2.0 mg·L-1 2,4-D) in medium on callus induction of Isodon amethystoides (Benth.) H. Hara were studied by using single-factor and L9(32) orthogonal experiments. The results show that compared with callus induction from leaves, calli can be induced from stem segments within a short time, which are loose and large in quantity. The results of orthogonal experiment show that the callus induction rate of stem segments of I. amethystoides varies from 72% to 93% in different media, in which, mass concentration of 2,4-D has a significant (P<0.05) effect on callus induction rate, while that of 6-BA has no significant effect. According to the results, MS medium (containing 30 g·L-1 sucrose and 7 g·L-1 agar, pH 5.8) with 0.1 mg·L-1 6-BA and 1.5 mg·L-1 2,4-D is considered as an optimum medium for callus induction from stem segments of I. amethystoides.
 

关键词香茶菜; 外植体类型; 激素浓度; 正交实验
Key wordsIsodon amethystoides (Benth.) H. Hara; explant type; hormone concentration; orthogonal experiment
作者刘芳1, 陶兴魁2, 张爱民3, 滕井通3, 丁盼盼3, 薛建平3
所在单位1. 宿州职业技术学院, 安徽 宿州 234101; 2. 宿州学院生物与食品工程学院, 安徽 宿州 234000; 3. 淮北师范大学生命科学学院, 安徽 淮北 235000
点击量434
基金项目安徽省高校省级优秀青年人才基金重点项目(2013SQRL151ZD); 淮北市人才培育计划(20140315); 宿州学院特色种植业苗种生产工程技术研究中心开放课题(2014XKF19); 安徽省高校自然科学研究项目(KJ2014B21)