研究了氮素和光照对甜菊（Stevia rebaudiana Bertoni）品种‘守田3号’（‘Shoutian No. 3’）和‘中山8号’（‘Zhongshan No. 8’）生长、氮素吸收以及叶片中甜菊糖苷含量和积累量的影响。结果表明：总体上看，同一光照处理下，与不施氮处理相比，施氮处理下2个甜菊品种单株地上部干质量、叶片叶绿素相对含量、叶片和茎中氮素含量、单株叶片和茎中氮素积累量以及单株总甜菊糖苷积累量显著升高，叶茎比、比叶质量、叶片氮素分配比例以及叶片中总甜菊糖苷和可溶性糖含量显著降低。同一氮素处理下，与正常光照处理相比，弱光处理下2个甜菊品种单株地上部干质量、叶茎比、比叶质量、叶片叶绿素相对含量及单株叶片和茎中氮素积累量总体上显著降低，叶片和茎中氮素含量总体上显著升高。不施氮处理下，与正常光照处理相比，弱光处理下甜菊品种‘中山8号’叶片中总甜菊糖苷和可溶性糖含量显著降低，甜菊品种‘守田3号’叶片中总甜菊糖苷和可溶性糖含量无显著变化；而施氮处理下，弱光处理对2个甜菊品种叶片中总甜菊糖苷和可溶性糖含量总体上无显著影响。与正常光照处理相比，弱光处理下2个甜菊品种单株总甜菊糖苷积累量显著降低。方差分析结果显示：氮素与光照的互作对甜菊单株地上部干质量、单株叶面积、单株叶片和茎中氮素积累量、叶片中总甜菊糖苷和可溶性糖含量以及单株总甜菊糖苷积累量有显著或极显著影响。综上所述，供试2个甜菊品种的干质量和氮素含量均受光照和氮素水平的影响，而叶片甜菊糖苷含量主要受氮素水平的调控。

 Effects of nitrogen and light on growth， nitrogen uptake， and leaf steviol glycosides content and accumulation of cultivar ‘Shoutian No. 3’ and ‘Zhongshan No. 8’ of Stevia rebaudiana Bertoni were studied. The results show that under the same light treatment， nitrogen application treatment significantly increases dry mass of above-ground part per plant， relative content of chlorophyll in leaves， nitrogen content in leaves and stems， nitrogen accumulation in leaves and stems per plant， and total steviol glycosides accumulation per plant of two S. rebaudiana cultivars in general compared with non-nitrogen application treatment， but significantly decreases leaf/stem ratio， specific leaf mass， leaf nitrogen allocation ratio， and contents of total steviol glycosides and soluble sugar in leaves. Under the same nitrogen treatment， weak light treatment significantly decreases dry mass of above-ground part per plant， leaf/stem ratio， specific leaf mass， relative content of chlorophyll in leaves， and nitrogen accumulation in leaves and stems per plant of two S. rebaudiana cultivars in general compared with normal light treatment， but significantly increases nitrogen content in leaves and stems in general. Under non-nitrogen application treatment， weak light treatment significantly decreases contents of total steviol glycosides and soluble sugar in leaves of cultivar ‘Zhongshan No. 8’ of S. rebaudiana compared with normal light treatment， but those indexes of cultivar ‘Shoutian No. 3’ of S. rebaudiana have no significant change； while under nitrogen application treatment， weak light treatment generally has no significant effect on those indexes of two S. rebaudiana cultivars. Compared with normal light treatment， total steviol glycosides accumulation per plant of two S. rebaudiana cultivars significantly decreases under weak light treatment. The variance analysis result shows that the interaction between nitrogen and light has significant or extremely significant effects on dry mass of above-ground part per plant， leaf area per plant， nitrogen accumulation in leaves and stems per plant， contents of total steviol glycosides and soluble sugar in leaves， and total steviol glycosides accumulation per plant of S. rebaudiana. In conclusion， dry mass and nitrogen content in two test S. rebaudiana cultivars are affected by light and nitrogen levels， while steviol glycosides content in leaves is mainly regulated by nitrogen level.