2019年10月17日 星期四
不同质量浓度多效唑对碗莲品种‘火花’生长和叶片部分生理指标的影响
Effects of paclobutrazol with different mass concentrations on growth and some physiological indexes of leaves of cultivar ‘Huohua’ of bowl lotus (Nelumbo nucifera)
2017年 第26卷 第3期 页码[51-58]    下载全文[0.9MB]  
摘要

研究了不同质量浓度(0~50 mg·L-1)多效唑对碗莲(Nelumbo nucifera Gaertn.)品种‘火花’(‘Huohua’)生长的影响及多效唑处理过程中叶片部分生理指标的变化,并进行了隶属函数值分析和相关性分析。结果表明:质量浓度10~50 mg·L-1多效唑处理抑制碗莲品种‘火花’生长。随着多效唑质量浓度提高,立叶数、立叶长、立叶宽、立叶高、花径、花高和开花总数总体上呈逐渐降低的趋势,且总体上与对照组(质量浓度0 mg·L-1多效唑)差异显著。随着处理时间延长,质量浓度10~50 mg·L-1多效唑处理组叶片中丙二醛(MDA)含量、过氧化物酶(POD)活性和多酚氧化酶(PPO)活性的变化趋势各异;质量浓度10~50 mg·L-1多效唑处理组叶片中可溶性蛋白质含量和超氧化物歧化酶(SOD)活性,以及质量浓度10、20和30 mg·L-1多效唑处理组叶片中过氧化氢酶(CAT)活性总体上呈先升高后降低的变化趋势,而质量浓度40和50 mg·L-1多效唑处理组叶片中CAT活性则呈逐渐降低的变化趋势。相关性分析结果表明:碗莲品种‘火花’生长指标的隶属函数值间以及叶片中可溶性蛋白质含量和POD活性的隶属函数值与生长指标的隶属函数值间的相关性总体在0.05或0.01水平上显著。研究结果显示:质量浓度10、20和30 mg·L-1多效唑处理可有效控制碗莲品种‘火花’生长,提高其观赏性;且其生长指标以及叶片中可溶性蛋白质含量和POD活性可以作为判断碗莲品种‘火花’矮化效果的可靠、简易指标。

Abstract

The effects of paclobutrazol with different mass concentrations (0-50 mg·L-1) on growth of cultivar ‘Huohua’ of bowl lotus (Nelumbo nucifera Gaertn.), and changes in some physiological indexes of leaves during paclobutrazol treatment period were studied, and analyses on subordinate function value and correlation were conducted. The results show that the paclobutrazol treatments with mass concentration of 10-50 mg·L-1 inhibit the growth of cultivar ‘Huohua’ of bowl lotus. With increasing of mass concentration of paclobutrazol, number, length, width and height of standing leaves, diameter and height of flowers, and total flower number generally decrease gradually, and in general, there is a significant difference with the control group (paclobutrazol with mass concentration of 0 mg·L-1). With prolonging of treatment time, change trends in malondialdehyde (MDA) content, peroxidase (POD) activity and polyphenol oxidase (PPO) activity in leaves in paclobutrazol treatment groups with mass concentration of 10-50 mg·L-1 are different. Soluble protein content and superoxide dismutase (SOD) activity in leaves in paclobutrazol treatment groups with mass concentration of 10-50 mg·L-1, and catalase (CAT) activity in leaves in paclobutrazol treatment groups with mass concentration of 10, 20 and 30 mg·L-1 generally increase at first and then decrease, while CAT activity in leaves in paclobutrazol treatment groups with mass concentration of 40 and 50 mg·L-1 shows a tendency to decrease gradually. The correlation analysis result shows that the correlation among subordinate function values of growth indexes, and the correlation of subordinate function values of soluble protein content and POD activity in leaves with those of growth indexes of cultivar ‘Huohua’ of bowl lotus are generally significant at 0.05 or 0.01 level. It is suggested that paclobutrazol treatments with mass concentration of 10, 20 and 30 mg·L-1 can effectively control the growth of cultivar ‘Huohua’ of bowl lotus, and improve its ornamental property. The growth indexes, soluble protein content and POD activity in leaves could be used as reliable and simple indicators to judge the dwarf effect of cultivar ‘Huohua’ of bowl lotus.

关键词碗莲品种‘火花’; 多效唑; 生长; 生理指标; 隶属函数值分析; 相关性分析
Key wordscultivar ‘Huohua’ of bowl lotus (Nelumbo nucifera Gaertn.); paclobutrazol; growth; physiological index; subordinate function value analysis; correlation analysis
作者李欣, 江君, 徐君, 朱建华, 姜红卫
所在单位苏州市农业科学院, 江苏 苏州 215155
点击量541
基金项目江苏省自然科学基金资助项目(BK20151229); 苏州市科技计划项目(SNG201508)