2024年7月25日 星期四
衍生化条件的优化及不同产地青蒿中青蒿素含量的柱前衍生化-HPLC 法测定
Optimization of derivation condition and determination of artemisinin content in Artemisia annua from different locations by pre-column derivation-HPLC method
2011年 第20卷 第1期 页码[88-90]    下载全文[0.8MB]  
Abstract

Derivation conditions of pre-column derivation-HPLC method used for determinating artemisinin content were compared and selected, and artemisinin content in above-ground part of Artemisia annua L. from seventeen locations was compared by optimal pre-column derivation-HPLC method. The optimal derivation condition is selected via comparing of 0. 2% NaOH solution addition (3, 4, 5, 6 and 7 mL), derivation temperature (30 ℃, 35 ℃, 40 ℃, 45 ℃and 50 ℃) and derivation time (0, 2, 5, 10, 20, 40 and 60 min) during derivation process. The optimal derivation condition is adding 5 mL 0. 2% NaOH solution, then insulating in 40 ℃water bath for 10 min. Differences of artemisinin content in A. annua from seventeen locations are obvious, the general trend is that artemisinin content decreases gradually with location from south to north, in which artemisinin content in A. annua from Youyang of Chongqing is the highest with a value of 7. 08 mg·g-1 . The method is simple and accurate with good reproducibility, and can be used to determinate artemisinin content in medicinal material of A. annua.

关键词青蒿; 青蒿素; 柱前衍生化-HPLC 法; 优化; 产地
Key wordsArtemisia annua L.; artemisinin; pre-column derivation-HPLC method; optimization; location
作者张伟, 秦民坚, 王国凯
所在单位中国药科大学中药资源学研究室教育部现代中药研究重点实验室, 江苏南京211198
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