对人参果(Solanum muricatum Ait)茎尖进行离体培养及快速繁殖。结果表明:适于外植体生长分化的培养基是不加任何激素或只加0.2mg·L^-1NAA的MS培养基;用MS+0.5mg·L^-1NAA培养基能促进组培苗茎尖快速伸长,有利于茎尖的剥离和脱毒培养;适于茎尖愈伤组织形成和分化的培养基为MS+0.2mg·L^-1NAA+2.0mg·L^-1 6-BA;适于壮苗快繁的培养基为MS+0.5mg·L^-1NAA+0.1mg·L^-1PP333。生物学和电镜检测结果表明,利用0.2～0.3mm茎尖培养的人参果试管苗已脱除病毒。

The culture and rapid propagation of shoot tips of Solanum muricatum Ait were investigated. The results showed that MS medium with both no phytohormone or 0.2 mg·L^-1 NAA was suitable to grow for explants. MS medium with 0.5 mg·L-1 NAA was suitable for rapidly lengthening of shoot tips and to isolate and culture of the shoot tips. The better medium for inducing and differentiating of callus was MS+ 0.2mg·L^-1 NAA+ 2.0mg·L^-1 6-BA, and the suitable medium for growth and rapid propagation was MS+ 0.5 mg·L^-1 NAA+ 0.1 mg·L^-1 PP₃₃₃. The results of examination by the methods of biology and electron microscope showed that there were no viruses in the plantlets cultured from the shoot tips of 0.2- 0.3 mm.