利用分光光度计比色测定大戟科乌桕(Sapium sebiferum Roxb.)、大戟(Euphorbia pekinensis Rupr.)、泽漆(Euphorbia helioscopia Lével)、重阳木(Bischofia polycarpam Airy Shaw)4种药用植物新鲜叶和茎中内生真菌的纤维素酶、漆酶和多酚氧化酶活性,并比较它们的差异。结果表明:茎内的真菌漆酶活性和多酚氧化酶活性普遍比叶内真菌高;重阳木内生真菌的漆酶及多酚氧化酶活性比乌桕、大戟和泽漆的内生真菌高。不同植物以及同一植物不同部位的内生真菌的胞外酶活性存在较大差异,推测其机理可能与内生真菌的种类和宿主植物之间的相互关系等因素有关。从乌桕和重阳木茎内皮中筛选到的漆酶和多酚氧化酶活性较高的2个菌株有良好的应用前景。

The activities of three extracellular enzyme including cellulase, laccase and polyphenol oxidase of thirty-six strains of endophytic fungi isolated from four species of medicinal plants ( Sapium sebiferum Roxb. , Euphorbia pekinensis Rupr. , Euphorbia helioscopia Lével and Bischofia polycarpam Airy Shaw )in Euphorbiaceae were detected spectrophotometrically and compared. The results showed that the activities of laccase and polyphenol oxidase of endophytic fungi isolated from stems were higher than those from leaves; the activities of laccase and polyphenol oxidase isolated from Bischofia polycarpam were higher than those from other three plants. It suggested that there were great discrepancy at the extracellular enzyme activities of isolates from different plants or different sites of the same plant, which possibly related with the sorts of endophytes and the relationship between endophytes and host plants. The two strains isolated from Sapium sebiferum and Bischofia polycarpam with high activities of laccase and polyphenol oxidase have good application prospects.