摘要 | 采用ISSR分子标记技术,对采自我国8省区的30个斑茅〔Erianthus arundinaceus (Retz.) Jesw.〕无性系的分子多态性进行了分析,共扩增出87条多态带,平均每个引物扩增的多态带数为5.1条,多态性条带比率(PPB)为64.89%,多态信息量(PIC)为0.58,说明我国斑茅具有较丰富的遗传多样性。根据研究结果进行聚类和主成分分析,可将30个斑茅无性系归为4大类,同一地区的斑茅无性系基本聚在同一类,呈现出一定的地域性分布规律。 |
Abstract | Inter-Simple Sequence Repeat(ISSR)molecular markers were used to detect the genetic diversity among30 clones of Erianthus arundinaceus(Retz.) Jesw. collected from eight provinces and regions in China. Eighty-seven polymorphic bands were produced totally, average 5.1 bands from each pair of ISSR primers, average percentage of polymorphic bands was 64.89%, and mean polymorphism information content was 0.58,whichwere indicating the rich genetic diversity of E. arundinaceus in China. Based on the cluster and principal component analyses on the genetic characteristics, all the clones could be divided into 4 groups according to the nearest phylogenetic relationship. In most cases, the clones from the same region were in the same group and only the clones from few regions could not be clustered into the same group, which considerably presented the geographical distribution of genetic diversity of the tested clones. |
关键词 | 斑茅; ISSR; 分子多态性; |
Key words | Erianthus arundinaceus (Retz.) Jesw.; ISSR marker; molecular polymorphism |
作者 | 张木清1,洪艺珣1,李奇伟2,刘少谋2,张垂明2,杨荣仲1 |
所在单位 | 1.福建农林大学农业部甘蔗生理生态与遗传改良重点实验室,福建福州350002; 2.广州甘蔗糖业研究所,广东广州510316 |
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基金项目 | 国家"863"计划课题糖料新品种选育(2001AA241191); 国家自然科学基金(30170590)资助项目; |