2024年7月23日 星期二
炭化液对NaCl胁迫下高丹草种子萌发和幼苗生长的影响
Effects of carbonized solutions on seed germination and seedling growth of Sorghum bicolor × sudanense under NaCl stress
2024年 第33卷 第3期 页码[80-88]    下载全文[1.5MB]  
摘要

为探讨炭化液对NaCl胁迫下高丹草(Sorghum bicolor × sudanense)种子萌发和幼苗生长的影响,利用工程水生植物金鱼藻(Ceratophyllum demersum Linn.)和苦草〔Vallisneria natans (Lour.) Hara〕制备炭化液,研究了NaCl胁迫下2种炭化液及其稀释比例对高丹草种子萌发及幼苗生长和生理指标的影响。结果显示:在0~16 g·L-1 NaCl范围内,高丹草种子的发芽率、发芽势和发芽指数随着NaCl质量浓度的提高呈下降趋势,在16 g·L-1 NaCl条件下发芽率仅5.6%;种子萌发阶段的耐盐适应质量浓度、耐盐半致死质量浓度和耐盐极限质量浓度分别为4.00、8.03和15.06 g·L-1。在4 g·L-1 NaCl条件下,添加金鱼藻炭化液和苦草炭化液(稀释比例1∶15)对高丹草种子发芽势、发芽率和发芽指数的影响不显著,但总体上显著(P<0.05)促进了芽长和根长;且处理10和15 d,金鱼藻炭化液处理的芽长和根长分别较苦草炭化液处理显著增加。在8~16 g·L-1NaCl条件下,添加金鱼藻炭化液(稀释比例1∶15)总体上显著增加了高丹草种子的发芽势、发芽率、发芽指数、芽长和根长。在8 g·L-1 NaCl条件下,添加稀释比例1∶15和1∶5的金鱼藻炭化液均较对照显著增加了高丹草幼苗的株高、根长、茎长、叶长、总鲜质量、地上部鲜质量、地下部鲜质量和根冠比,显著降低了超氧阴离子含量、过氧化氢酶活性和可溶性蛋白质含量。综上所述,较高质量浓度NaCl胁迫下,添加金鱼藻炭化液显著促进了高丹草的种子萌发,有效减少了高丹草幼苗受到的氧化损伤,进而缓解了NaCl对高丹草种子萌发和幼苗生长的胁迫作用。

Abstract

 To investigate the effects of carbonized solutions on seed germination and seedling growth of Sorghum bicolor × sudanense under NaCl stress, the carbonized solutions were prepared by using engineering aquatic plants Ceratophyllum demersum Linn. and Vallisneria natans (Lour.) Hara, and the effects of two carbonized solutions and their dilution ratios on seed germination and growth and physiological indexes of seedlings of S. bicolor × sudanense under NaCl stress were explored. The results show that within the range of 0-16 g·L-1 NaCl, the germination rate, germination potential, and germination index of S. bicolor × sudanense seeds decrease with the increase of NaCl mass concentration, and the germination rate is only 5.6% under 16 g·L-1 NaCl condition; the salt tolerance adaptive mass concentration, salt tolerance semi-lethal mass concentration, and salt tolerance limit mass concentration at seed germination stage are 4.00, 8.03, and 15.06 g·L-1, respectively. Under 4 g·L-1 NaCl condition, the addition of carbonized solutions of C. demersum and V. natans (dilution ratio 1∶15) have no significant effects on the germination potential, germination rate, and germination index of S. bicolor × sudanense seeds, but significantly (P<0.05) promote the shoot length and root length in general; after 10 and 15 d of treatment, the shoot length and root length of the carbonized solution treatment of C. demersum increase significantly compared with those of the carbonized solution treatment of V. natans. Under 8-16 g·L-1 NaCl conditions, the addition of carbonized solution of C. demersum (dilution ratio 1∶15) significantly increases the germination potential, germination rate, germination index, shoot length, and root length of S. bicolor × sudanense seeds in general. Under 8 g·L-1 NaCl condition, the addition of carbonized solutions of C. demersum diluted at the ratios of 1∶15 and 1∶5 both significantly increase the height, root length, stem length, leaf length, total fresh mass, fresh mass of above-ground part, fresh mass of under-ground part, and root/shoot ratio of S. bicolor × sudanense seedlings compared with the control, and significantly decrease the superoxide anion content, catalase activity, and soluble protein content. In conclusion, under the relatively high mass concentrations of NaCl stress, the addition of carbonized solution of C. demersum significantly promote the germination of S. bicolor × sudanense seeds, effectively reduce the oxidative damage of S. bicolor × sudanense seedlings, and then alleviate the stress of NaCl on seed germination and seedling growth of S. bicolor × sudanense.

 

关键词高丹草; 炭化液; NaCl胁迫; 种子萌发; 幼苗生长
Key wordsSorghum bicolor × sudanense; carbonized solution; NaCl stress; seed germination; seedling growth
作者梁玉1, 李金凤1, 崔键1, 李文平2, 常雅军1, 汪帆2, 刘晓静1, 姚东瑞1
所在单位1. 江苏省中国科学院植物研究所(南京中山植物园) 江苏省植物资源研究与利用重点实验室 江苏省水生植物资源与水环境修复工程研究中心, 江苏 南京 210014; 2. 江苏沿海生态科技发展有限公司, 江苏 南京 211800
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基金项目江苏省碳达峰碳中和科技创新专项资金项目(BE2022304); 江苏省沿海开发集团有限公司“揭榜挂帅”项目(2022YHTDJB015)