对采自中国不同地区的6份狗牙根〔Cynodon dactylon(L.)Pers.〕优良选系进行了RAPD分子标记实验。选用15个引物共扩增出438条带,平均每个引物扩增出29条带,其中多态性带415条,多态性位点百分率达到59.02%～75.49%。各选系间遗传相似性系数差异较大(0.408～0.672),说明各选系间在DNA水平上存在着丰富的遗传多样性,与新品种C106(爬地青)相比,各选系还存在一定的改良空间。实验结果也表明,RAPD分子标记可成功地用于中国狗牙根优良选系遗传多样性的研究及品种鉴定。

The experiment of RAPD molecular marker was conducted on 6 merit selections of Cynodon dactylon(L.) Pers. in China. 438 bands were amplified by 15 primer sequences, 415 polymorphic bands were produced, average 29 bands from each primer, average percentage of polymorphic band of 6 selections reached to 59. 02% -75. 49%. The genetic similarity coefficients among 6 selections were obviously different(0. 408 -0. 672). The results suggested that there is abundant genetic diversity among different selections on DNA level. Compared with the new cultivar C106, the other selections can be improved further. Besides, the results also proved that the RAPD molecular marker can be successfully used to research the genetic diversity and new variety identification of C. dactylon.