Taking Eriobotrya japonica （Thunb.） Lindl. leaves from 12 localities in 10 provinces （autonomous regions） as research materials， HPLC method for determination of sesquiterpene glycoside A content in E. japonica leaves was established， and sesquiterpene glycoside A contents in E. japonica leaves from different localities were determined and compared. The results show that sesquiterpene glycoside A contents in E. japonica leaves from different localities are 0.613-1.412 mg·g^-1， and there are obvious differences among different localities. In which， sesquiterpene glycoside A content in E. japonica leaves from Wuzhou of Guangxi locality is the highest， that in E. japonica leaves from Kaifeng of He^,nan locality is the lowest， and there is a significant difference between these two localities. It is suggested that Wuzhou of Guangxi is superior locality of E. japonica leaves.