2024年4月19日 星期五
鸡蛋果SUT基因家族的鉴定及表达分析
Identification and expression analysis on SUT gene family of Passiflora edulis
2023年 第32卷 第3期 页码[33-44]    下载全文[4.3MB]  
摘要

采用生物信息学方法,从鸡蛋果(Passiflora edulis Sims)全基因组中鉴定SUT基因家族成员,并对其编码蛋白的理化性质、亚细胞定位、三级结构和系统发育以及基因结构、共线性和表达模式进行了分析。结果显示:鸡蛋果全基因组中鉴定出的5个PeSUT基因定位在4条染色体上,绝大多数SUT基因家族成员含有8或9个外显子,其编码蛋白的氨基酸残基数介于328~1 014之间,均定位于质膜。鸡蛋果5个SUT家族成员分为3个亚族。鸡蛋果SUT基因家族中PeSUT1和PeSUT5基因以及PeSUT2和PeSUT3基因存在共线关系。鸡蛋果3个SUT基因与拟南芥〔Arabidopsis thaliana (Linn.) Heynh.〕4个SUC基因间存在共线关系。鸡蛋果SUT基因家族启动子中存在大量与激素和逆境胁迫响应相关的顺式作用元件。转录组数据分析结果表明:PeSUT1和PeSUT2基因在鸡蛋果品种‘台农1号’(‘Tainong No. 1’)和‘黄金果’(‘Huangjinguo’)叶片和果皮中表达量较低或不表达;PeSUT3基因在‘台农1号’叶片中的表达量低于‘黄金果’叶片,但在‘台农1号’果皮中的表达量却高于‘黄金果’果皮;PeSUT4基因在‘台农1号’叶片和果皮中的表达量分别高于‘黄金果’叶片和果皮;PeSUT5基因在‘台农1号’和‘黄金果’叶片中的表达量基本相同,在‘台农1号’果皮中的表达量低于‘黄金果’果皮。实时荧光定量PCR分析结果表明:PeSUT3基因在低温(4 ℃)胁迫处理下的相对表达量较正常温度(25 ℃)处理极显著(P≤0.01)上调。综合分析结果表明: PeSUT3基因可能参与鸡蛋果叶片对低温胁迫的响应。

Abstract

The SUT gene family members were identified from whole genome of Passiflora edulis Sims by using bioinformatics methods, and physicochemical properties, subcellular localization, tertiary structure, and phylogeny of their encoded proteins, gene structure, collinearity, and expression pattern were analyzed. The results show that five PeSUT genes identified from whole genome of P. edulis are located on four chromosomes, most SUT gene family members contain eight or nine  exons, the number of amino acid residues of their encoded proteins are within 328-1 014, and they are all located on plasma membrane. Five SUT family members of P. edulis are divided into three sub-families. There are collinear relationships between PeSUT1 and PeSUT5 genes and between PeSUT2 and PeSUT3 genes in SUT gene family of P. edulis. There are collinear relationships between three SUT genes of P. edulis and four SUC genes of Arabidopsis thaliana (Linn.) Heynh. There are a large number of cis-acting elements related with hormone and stress response in promoters of SUT gene family of P. edulis. The transcriptomic data analysis result shows that the expression of PeSUT1 and PeSUT2 genes in leaves and pericarps of cultivar ‘Tainong No. 1’ and ‘Huangjinguo’ of P. edulis is low or not expressed; the expression of PeSUT3 gene in leaves of ‘Tainong No. 1’ is lower than that in leaves of ‘Huangjinguo’, but is higher in pericarps of ‘Tainong No. 1’ than that in pericarps of ‘Huangjinguo’; the expression of PeSUT4 gene in leaves and pericarps of ‘Tainong No. 1’ is higher than those in leaves and pericarps of ‘Huangjinguo’, respectively; the expression of PeSUT5 gene in leaves of ‘Tainong No. 1’ and ‘Huangjinguo’ is basically the same, but the expression in pericarps of ‘Tainong No. 1’ is lower than that in pericarps of ‘Huangjinguo’. The realtime fluorescence quantitative PCR analysis result shows that the relative expression of PeSUT3 gene under low temperature (4 ℃) stress treatment is extremely significantly (P≤0.01) up-regulated compared with that under normal temperature (25 ℃) treatment. The comprehensive analysis result shows that PeSUT3 gene may be involved in the response of P. edulis leaves to low temperature.

关键词鸡蛋果; 蔗糖转运蛋白(SUTs); 基因家族; 表达; 低温胁迫
Key wordsPassiflora edulis Sims; sucrose transporters (SUTs); gene family; expression; low temperature stress
作者李韵1a,1b, 刘昱君1a,1b, 潘若云1a,1b, 魏秀清1a,1b,2, 曾黎辉1a,1b, 方庭1a,1b
所在单位1. 福建农林大学: a. 园艺学院, b. 园艺植物遗传育种研究所, 福建 福州 350002; 2. 福建省农业科学院果树研究所, 福建 福州 350013
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基金项目福建省自然科学基金项目(2021J05022); 福建省种业创新与产业化工程项目(zycxny2021010); 福建省高原学科建设经费(102/71201801101); 福建省公益类科研院所专项(2019R1028-9)