2024年2月28日 星期三
迷迭香叶中三萜酸类成分及其抑菌活性分析
Analyses on triterpene acids components in leaves of Rosmarinus officinalis and their inhibitory activities
2019年 第28卷 第1期 页码[115-117]    下载全文[1MB]  
Abstract

Triterpene acids components in leaves of Rosmarinus officinalis Linn. were separated and identified by HPLC, silica gel column chromatography and reversedphase C18 semi-preparative liquid chromatography method. And taking the minimum inhibitory concentration (MIC) as the index, the inhibitory activity of each compound was determined. The results show that 4 triterpene acids compounds are isolated from ethanol extracts of leaves of R. officinalis; according to the peak characteristics of reference substances, compound 2, 3 and 4 are identified as betulinic acid, oleanolic acid and ursolic acid, respectively; based on physicochemical constants and spectral data, compound 1 is identified as Δ20(30)-ene-ursolic acid. Ursolic acid, Δ20(30)-ene-ursolic acid and oleanolic acid have a certain inhibitory activity to Gram-positive strains (methicillin-resistant Staphylococcus aureus, MRSA), in which, the MIC value of ursolic acid to MRSA is 8.000-128.000 μg·mL-1, and its inhibitory activity is the strongest; however, none of 4 compounds shows inhibitory activity to Gramnegative strain (Escherichia coli S002).

关键词迷迭香叶; 三萜酸类; Δ20(30)-烯-熊果酸; 熊果酸; 最低抑菌浓度
Key wordsleaves of Rosmarinus officinalis Linn.; triterpene acids; Δ20(30)-ene-ursolic acid; ursolic acid; the minimum inhibitory concentration
作者李菌芳, 王一旻, 袁干军, 李国华, 许雪杰, 宋晓媛
所在单位江西农业大学生物科学与工程学院, 江西 南昌 330045
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基金项目国家自然科学基金资助项目(81260476; 81460529); 国家级大学生创新创业训练计划项目(201610410023)