摘要 | 为了研究费菜内生细菌对其根部活性成分积累的潜在机制,利用前期从野生费菜〔Phedimus aizoon (Linn.)'t Hart〕中分离得到的3株具有促生潜力的内生细菌,以株龄1 a费菜扦插苗为实验材料,测定目标菌株回接后费菜根部活性成分含量,分析目标菌株接种后费菜中差异表达基因,并对与促生和活性成分合成相关的差异表达基因进行实时荧光定量逆转录PCR(qRT-PCR)分析。结果显示:与未接菌处理相比,接种内生细菌菌株Pwx052、 Pwx053和Pwx056处理费菜根部总黄酮、总多糖和总三萜的含量极显著(p<0.01)升高,但仅接种菌株Pwx053处理的三七皂苷含量极显著升高。根据分子鉴定结果,初步鉴定菌株Pwx053为天蓝黄链霉菌(Streptomyces coelicoflavus)。转录组分析结果显示:接种菌株Pwx053的费菜转录组中上调差异表达基因富集的通路与植物防御反应、活性成分合成、细胞发育等有关。qRT-PCR分析结果显示:接种菌株Pwx053处理费菜根部与活性成分合成相关的CM3、HMGL等差异表达基因的表达呈上调趋势。上述研究结果表明:菌株Pwx053处理费菜后引起植物防御反应,并上调与活性成分合成相关基因表达,从而促进费菜活性成分的积累,说明菌株Pwx053具有开发为微生物菌肥的潜力。
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Abstract | To investigate the potential mechanisms of endophytic bacteria from Phedimus aizoon (Linn.)'t Hart on the accumulation of active components in the roots of P. aizoon, three endophytic bacterial isolates with growth-promoting potential, which were previously isolated from wild P. aizoon, were utilized, and one-year-old cutting seedlings of P. aizoon were taken as the experimental material. The contents of active components in the roots of P. aizoon were measured after re-inoculation of the target bacterial isolate, differentially expressed genes in P. aizoon after inoculation of the target isolate were analyzed, and real-time fluorescent quantitative reverse transcription PCR (qRT-PCR) was performed for differentially expressed genes related to growth promotion and active component synthesis. The results show that compared with uninoculated treatment, the contents of flavonoids, polysaccharides, and triterpenes in the roots of P. aizoon inoculated with endophytic bacterial isolates Pwx052, Pwx053, and Pwx056 are extremely significantly (p<0.01) increased, but only the content of notoginsenoside inoculated with isolate Pwx053 is extremely significantly increased. Based on the result of molecular identification, isolate Pwx053 is preliminarily identified as Streptomyces coelicoflavus. The transcriptome analysis result shows that the enriched pathways of up-regulated differentially expressed genes in the transcriptome of P. aizoon inoculated with isolate Pwx053 are related to plant defense response, active component synthesis, cell development, etc. The qRT-PCR analysis result shows that the expression of differentially expressed genes related to active component synthesis, such as CM3 and HMGL, exhibits an up-regulation tendency in the roots of P. aizoon inoculated with isolate Pwx053. It is suggested that treatment of P. aizoon with isolate Pwx053 triggers plant defense response and up-regulates the expression of genes related to active component synthesis, thereby promoting the accumulation of active components in P. aizoon, indicating that isolate Pwx053 has the potential to be developed as microbial fertilizer.
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关键词 | 费菜; 内生细菌; 活性成分; 转录组 |
Key words | Phedimus aizoon (Linn.)'t Hart; endophytic bacterium; active component; transcriptome |
作者 | 蒋侄丽, 傅海兰, 王艺璇, 李艳婷, 刘明杰, 吴宣, 蒋继宏, 李露丹 |
所在单位 | 江苏师范大学生命科学学院 江苏省药食植物生物技术国家重点实验室(培育), 江苏 徐州 221116 |
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下载次数 | 1110 |
基金项目 | 国家自然科学基金青年基金项目(32101519); 江苏省自然科学基金青年基金项目(BK20210919); 江苏省研究生科研与实践创新计划项目(KYCX24_3020) |