为提高盾叶薯蓣(Dioscorea zingiberensis C.H. Wright)中薯蓣皂甙元得率, 在实验室条件下, 以薯蓣皂甙元含量为评价指标, 采用 4因素 4水平的正交试验, 用高效液相色谱法对 16种提取方法所得的薯蓣皂甙元含量进行了比较分析。结果表明, 硫酸的浓度对薯蓣皂甙元含量有极显著影响, 在实验室条件下,可采用 10g样品加 2. 0mol·L^-1硫酸 200mL水解 4h,水解物用石油醚回流提取 5h,能快速准确提取盾叶薯蓣中的薯蓣皂甙元。

To increase the extract rate of diosgenin in rhizome of Dioscorea zingiberensis C.H. Wright, several important factors were analyzed in the orthogonal study of four factors and four levels, the content of diosgenin was valued as an evaluation index using RP-HPLC. The results indicate that the concentration of sulphuric acid has an obvious effect on the content of diosgenin extracted. So in the condition of laboratory, 10 g sample is added in 200mL2.0 mol·L^-1 H₂SO₄ and is hydrolyzed for 4h, then is refluxed for 5h with petroleum ether, which can be used as a rapidly extraction way.