2024年2月28日 星期三
以α-葡萄糖苷酶活性抑制率为指标优化山茱萸果肉的提取条件
Optimization of extraction condition of Cornus officinalis flesh in taking inhibition rate of α-glucosidase activity as index
2013年 第22卷 第1期 页码[112-114]    下载全文[0.7MB]  
Abstract

Taking an inhibition rate ofα-glucosidase activity as index, four factors including solid-liquid ratio, volume ratio of ethanol, time and temperature of ultrasonic treatment were selected to carry out single factor and orthogonal L9 (34 ) experiments, extraction condition of Cornus officinalis Sieb. et Zucc. flesh were optimized and selected. The results show that ultrasonic treatment temperature is the most factor related to inhibition rate of ethanol extract from C. officinalis flesh toα-glucosidase. The optimization extraction condition is solid-liquid ratio 1︰15 (W V), 50% ethanol (volumn ratio),ultrasonic treatment temperature 70 ℃and ultrasonic treatment time 30 min. And inhibition rate of flesh extract obtained under the optimization extraction condition is the highest (98. 5%). The hemi-inhibitory concentration (IC50 ) of extract from C. officinalis flesh toα-glucosidase is 409. 30 mg·L-1 , which is much more lower than that of the control of acarbose (890. 48 mg·L-1 ).

关键词山茱萸果肉; 乙醇提取物; 提取条件; α-葡萄糖苷酶活性; 抑制率; 正交实验
Key wordsCornus officinalis Sieb. et Zucc. flesh; ethanol extract; extraction condition; α-glucosidase activity; inhibition rate; orthogonal experiment
作者李慧敏, 刘艳妮, 王小蕊, 康杰芳
所在单位陕西师范大学教育部药用资源与天然药物化学教育部重点实验室西北濒危药材资源开发国家工程实验室生命科学学院, 陕西西安710062
点击量1415
下载次数831
基金项目国家“十二五”科技支撑计划项目(2011BA106B06); 西安市科技创新支撑计划项目[CXY1018(3)]